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. 2017 Feb 23;61(3):e02300-16.
doi: 10.1128/AAC.02300-16. Print 2017 Mar.

PER-8, a Novel Extended-Spectrum β-Lactamase PER Variant, from an Acinetobacter baumannii Clinical Isolate in Nepal

Tatsuya Tada  1 , Shovita Shrestha  2 , Kayo Shimada  1 , Hiroshi Ohara  3 , Jeevan B Sherchand  2 , Bharat M Pokhrel  4 , Teruo Kirikae  5
Affiliations

PER-8, a Novel Extended-Spectrum β-Lactamase PER Variant, from an Acinetobacter baumannii Clinical Isolate in Nepal

Tatsuya Tada et al. Antimicrob Agents Chemother. .

Abstract

A novel PER-type extended-spectrum β-lactamase, PER-8, was identified in an Acinetobacter baumannii clinical isolate obtained in Nepal. The amino acid sequence of PER-8 has a substitution at position 39 (Gly to Glu) compared with that of PER-7. The kcat/Km ratio of PER-8 for aztreonam was lower than that of PER-7, while the kcat/Km ratio of PER-8 for imipenem was higher than that of PER-7. The genomic environment surrounding blaPER-8 was intI1 blaPSE-1qacEDI sulI ISCR1-blaPER-8gts sulI orfX on a 100-kb plasmid.

Keywords: Acinetobacter baumannii; PER-type ESBLs; plasmid-mediated resistance.

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Figures

FIG 1
FIG 1
Genetic environments surrounding blaPER genes in A. baumannii IOMTU442 (GenBank accession no. AB985401), IOMTU448 (GenBank accession no. LC020101), IOMTU433 (GenBank accession no. AP014650) (22), and AP2 (GenBank accession no. HQ713678) (17). The blaPER-7 gene in A. baumannii AP2 was located on the chromosome, whereas the blaPER-7 gene in A. baumannii IOMTU433 and the blaPER-8 gene in A. baumannii IOMTU442 were located on plasmids.
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