Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Mar 1;54(2):387-395.
doi: 10.1093/jme/tjw187.

Impact of Autocidal Gravid Ovitraps on Chikungunya Virus Incidence in Aedes aegypti (Diptera: Culicidae) in Areas With and Without Traps

Roberto Barrera  1 , Veronica Acevedo  1 , Gilberto E Felix  1 , Ryan R Hemme  1 , Jesus Vazquez  2 , Jorge L Munoz  2 , Manuel Amador  1
Affiliations

Impact of Autocidal Gravid Ovitraps on Chikungunya Virus Incidence in Aedes aegypti (Diptera: Culicidae) in Areas With and Without Traps

Roberto Barrera et al. J Med Entomol. .

Erratum in

  • Corrigendum.
    [No authors listed] [No authors listed] J Med Entomol. 2017 Jul 1;54(4):1098. doi: 10.1093/jme/tjx034. J Med Entomol. 2017. PMID: 28431125 No abstract available.

Abstract

Puerto Rico detected the first confirmed case of chikungunya virus (CHIKV) in May 2014 and the virus rapidly spread throughout the island. The invasion of CHIKV allowed us to observe Aedes aegypti (L.) densities, infection rates, and impact of vector control in urban areas using CDC autocidal gravid ovitraps (AGO traps) for mosquito control over several years. Because local mosquitoes can only get the virus from infectious residents, detecting the presence of virus in mosquitoes functions as a proxy for the presence of virus in people. We monitored the incidence of CHIKV in gravid females of Ae. aegypti in four neighborhoods-two with three AGO traps per home in most homes and two nearby neighborhoods without AGO mosquito control traps. Monitoring of mosquito density took place weekly using sentinel AGO traps from June to December 2014. In all, 1,334 pools of female Ae. aegypti (23,329 individuals) were processed by real-time reverse transcription PCR to identify CHIKV and DENV RNA. Density of Ae. aegypti females was 10.5 times lower (91%) in the two areas with AGO control traps during the study. Ten times (90.9%) more CHIKV-positive pools were identified in the nonintervention areas (50/55 pools) than in intervention areas (5/55). We found a significant linear relationship between the number of positive pools and both density of Ae. aegypti and vector index (average number of expected infected mosquitoes per trap per week). Temporal and spatial patterns of positive CHIKV pools suggested limited virus circulation in areas with AGO traps.

Keywords: Aedes aegypti; arboviral transmission; invasive species; mosquito control; vector-borne pathogen.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Average number of female Aedes aegypti per sentinel AGO trap, CHIKV-positive mosquito pools, and accumulated rainfall (third and second week before sampling) per week in non-intervention (Playa, Arboleda) and intervention areas (La Margarita, Villodas) during the second half of 2014 in Puerto Rico.
Figure 2.
Figure 2.
Average number of female Aedes aegypti per trap per week in intervention (solid dots) and non-intervention (open dots) areas in Puerto Rico from June to December 2014. The dotted line drawn at 3 females per trap per week separates most average captures between intervention and non-interventions areas.
Figure 3.
Figure 3.
Map of Playa community showing streets and locations of traps with CHIKV-positive mosquito pools from June to December 2014 in southern Puerto Rico.
Figure 4.
Figure 4.
Map of Arboleda community showing streets and locations of traps with CHIKV-positive mosquito pools from June to December 2014 in southern Puerto Rico.
Figure 5.
Figure 5.
Relationship between number of CHIKV-positive mosquito pools and average number of female Aedes aegypti per trap per week in all study areas from June to December 2014.
See this image and copyright information in PMC

References

    1. Agarwal A, Dash PK, Singh AK, Sharma S, Gopalan N, Rao PVL, Parida MM, and Reiter P. 2014. Evidence of experimental vertical transmission of emerging novel ECSA genotype of chikungunya virus in Aedes aegypti. PLoS Negl Trop Dis 8(7): e2990. doi: 10.1371/journal.pntd.0002990. - DOI - PMC - PubMed
    1. Aliota MT, Walker EC, Uribe Yepes A, Dario Velez I, Christensen BM, and Osorio JE. 2016. The wMel strain of Wolbachia reduces transmission of chikungunya virus in Aedes aegypti. PLoS Negl Trop Dis 10: e0004677. - PMC - PubMed
    1. Alphey L, McKemey A, Nimmo D, Neira Oviedo M, Lacroix R, Matzen K, and Beech C. 2013. Genetic control of Aedes mosquitoes. Pathog Glob Health 107: 170–179. - PMC - PubMed
    1. Barrera R 2010. Dinamica del dengue y Aedes aegypti en Puerto Rico. Biomedica 21: 179–195.
    1. Barrera R 2015. Considerations for disrupting dengue virus transmission: Ecology of Aedes aegypti and current (non genetic) methods of control, pp. 103–124. In Adelman ZN (ed.), Genetic control of malaria and dengue. Academic Press, Oxford.