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. 1989 Aug 1;261(3):787-92.
doi: 10.1042/bj2610787.

Low-Mr iron isolated from guinea pig reticulocytes as AMP-Fe and ATP-Fe complexes

J Weaver  1 , S Pollack
Affiliations

Low-Mr iron isolated from guinea pig reticulocytes as AMP-Fe and ATP-Fe complexes

J Weaver et al. Biochem J. .

Abstract

Guinea pig reticulocytes were pulse-labelled with 59Fe bound to transferrin. Haemolysates prepared from these reticulocytes were subjected to rapid (NH1)2SO1 precipitation and then chromatography on an anion-exchange resin. ATP-bound 59Fe was the dominant species in the reticulocyte cytosol; 2,3-bisphosphoglycerate and GTP iron complexes were not detected despite the fact that these were stable with (NH1)2SO1 precipitation and readily detected with anion-exchange chromatography. AMP-bound Fe was a minor component of the cytosol following rapid (NH1)2SO4 precipitation, and the major component when iron was released from transferrin by haemolysates. We speculate that ATP-Fe may be degraded in the cell to permit utilization of its iron for haem synthesis.

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References

    1. J Clin Invest. 1968 Jul;47(7):1473-82 - PubMed
    1. Free Radic Biol Med. 1988;4(3):185-98 - PubMed
    1. Proc Soc Exp Biol Med. 1972 Apr;139(4):1379-84 - PubMed
    1. Am J Physiol. 1972 Jun;222(6):1628-33 - PubMed
    1. Acta Haematol. 1972;47(3):157-63 - PubMed