doi: 10.1042/bj2620659.
Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor
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- PMID: 2803273
- PMCID: PMC1133318
- DOI: 10.1042/bj2620659
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Identification of a novel autophosphorylation site (P4) on the epidermal growth factor receptor
Biochem J.
.
Abstract
Three major autophosphorylation sites are located near the C-terminus of the epidermal growth factor receptor, but a fourth site is repeatedly detected. We report here the purification and sequencing of a tryptic peptide containing this site, Tyr-1086. Furthermore, we demonstrate that additional phosphopeptides are observed following both partial digestion and overdigestion. Finally, we show that Tyr-1086 can be phosphorylated in intact cells.
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