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. 2016 Dec 29;22(1):50.
doi: 10.3390/molecules22010050.

Formation and Physiochemical Properties of Silver Nanoparticles with Various Exopolysaccharides of a Medicinal Fungus in Aqueous Solution

Wenjie Jian  1   2 , Lu Zhang  3 , Ka-Chai Siu  4 , Angxin Song  5 , Jian-Yong Wu  6
Affiliations

Formation and Physiochemical Properties of Silver Nanoparticles with Various Exopolysaccharides of a Medicinal Fungus in Aqueous Solution

Wenjie Jian et al. Molecules. .

Abstract

Natural polysaccharides are the most widely used biopolymers for green synthesis of eco-friendly silver nanoparticles (AgNPs). In a previous study, a high molecular weight (MW) fraction of exopolysaccharides (EPS) produced by a medicinal fungus Cs-HK1 has been shown useful for green and facile synthesis of AgNPs in water. This study was to further evaluate the effects of molecular properties of EPS on the formation, stability and properties of AgNPs with different EPS fractions at various pH conditions. Three EPS fractions (P0.5, P2.0 and P5.0: MW high to low and protein content low to high) were reacted with silver nitrate at various pH 3.0-8.0 in water. The most favorable pH range was 5.5-8.0 for the formation and stable dispersion of AgNPs. At a given pH, the maximum amount of AgNPs was produced with P5.0, and the minimum with P0.5. The shape, size and physiochemical properties of AgNPs were strongly affected by the molecular characteristics of EPS (MW and conformation). The results may be helpful for understanding the factors and mechanisms for formation of stable AgNPs with natural polysaccharides and the interactions between AgNPs and the polysaccharide hydrocolloids in water.

Keywords: dispersion stability; fungal exopolysaccharide; molecular characteristics; molecular interactions; pH; silver nanoparticle.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
UV-Vis absorbance spectra of AgNO3 and (a) P0.5; (b) P2.0; (c) P5.0 mixture reaction solution at various pH value after thermal treatment for 240 min; and peak absorbance at UV-Vis light absorbance spectra of AgNO3; and (d) P0.5; (e) P2.0; (f) P5.0 mixture reaction solution at various pH value for various periods of reaction time.
Figure 2
Figure 2
Transmission electron microscopy of (a) P0.5-AgNPs; (b) P2.0-AgNPs; and (c) P5.0-AgNPs at different pH. (AgNP formation did not occur at pH = 4 for EPS fractions P0.5 and P2.0).
Figure 3
Figure 3
Hydrodynamic radius of EPS fractions (P0.5, P2.0, P5.0) and EPS-AgNP complexes (P0.5-AgNPs, P2.0-AgNPs, P5.0-AgNPs) at different pH. (AgNP formation did not occur at pH = 4 for EPS fractions P0.5 and P2.0).
Figure 4
Figure 4
Photographs of EPS-AgNP dispersions at pH 4.0, pH 5.5, pH 7.0, and pH 8.0 after stored at room temperature for 2 months. (AgNP formation did not occur at pH = 4 for EPS fractions P0.5 and P2.0).
Figure 5
Figure 5
Schematic model for formation of EPS-AgNP complexes.
See this image and copyright information in PMC

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