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Review
. 2017 Jun;21(3):259-268.
doi: 10.1007/s40291-016-0251-y.

The Importance of Standardization on Analyzing Circulating RNA

Inyoul Lee  1 , David Baxter  1 , Min Young Lee  1 , Kelsey Scherler  1 , Kai Wang  2
Affiliations
Review

The Importance of Standardization on Analyzing Circulating RNA

Inyoul Lee et al. Mol Diagn Ther. 2017 Jun.

Abstract

Circulating RNAs, especially microRNAs (miRNAs), have recently emerged as non-invasive disease biomarkers. Despite enthusiasm and numerous reports on disease-associated circulating miRNAs, currently there is no circulating miRNA-based diagnostic in use. In addition, there are many contradictory reports on the concentration changes of specific miRNA in circulation. Here we review the impact of various technical and non-technical factors related to circulating miRNA measurement and elucidate the importance of having a general guideline for sample preparation and concentration measurement in studying circulating RNA.

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Conflict of interest statement

Conflict of Interest statement

The authors (Kai Wang, Inyoul Lee, David Baxter, Min Young Lee, and Kelsey Scherler) declare that they have no competing interests.

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