Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jan;32(1):41-46.
doi: 10.5001/omj.2017.08.

Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium

Jeyasakthy Saniasiaya  1 , Rosdan Salim  1 , Irfan Mohamad  1 , Azian Harun  2
Affiliations

Antifungal Effect of Malaysian Aloe vera Leaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium

Jeyasakthy Saniasiaya et al. Oman Med J. 2017 Jan.

Abstract

Objectives: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of Malaysian Aloe vera leaf extract on otomycosis species including Aspergillus niger and Candida albicans.

Methods: This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of Malaysian Aloe vera leaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC).

Results: For A. niger, a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts of Aloe vera leaf for C. albicans. The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL for A. niger and since no zone of inhibition was obtained for C. albicans the MIC was not determined.

Conclusions: The antifungal effect of alcohol extracts of Malaysian Aloe vera leaf is better than the aqueous extract for A. niger (p < 0.001). Malaysian Aloe vera has a significant antifungal effect towards A. niger.

Keywords: Aloe vera; Aspergillus Niger; Candida Albicans; Otomycosis.

PubMed Disclaimer

Figures

Figure 1
Figure 1
(a) Aloe vera leaves dried at 45 ºC in a hot air oven. (b) Soxhlet apparatus used for extraction. (c) Candida albicans and Aspergillus niger culture.
Figure 2
Figure 2
Zones of inhibition of Aspergillus niger for five different aqueous extract Aloe vera leaf concentrations.
Figure 3
Figure 3
Determination of the MIC using a scattered plot graph of X versus log concentration.
See this image and copyright information in PMC

References

    1. Vennewald I, Klemm E. Otomycosis: Diagnosis and treatment. Clin Dermatol 2010. Mar;28(2):202-211. - PubMed
    1. Reynolds T, Dweck AC. Aloe vera leaf gel: a review update. J Ethnopharmacol 1999. Dec;68(1-3):3-37. - PubMed
    1. Radha MH, Laxmipriya NP. Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review. J Tradit Complement Med 2014. Dec;5(1):21-26. - PMC - PubMed
    1. Newall CA, Anderson LA, Philipson JD. Herbal medicines: A guide for health-care professionals. London: The Pharmaceutical Press. 1996;25.
    1. Kumar S, Yadav M, Yadav M, Yadav JP. Comparative analysis of antimicrobial activity of methanolic extracts of Aloe vera and quantification of Aloe Emodin collected from different climatic zones of India. A C Microb. 2015;(6)2:1.

LinkOut - more resources