Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Winter;18(4):582-587.
doi: 10.22074/cellj.2016.4724. Epub 2016 Sep 26.

Oxidative Stress Statues in Serum and Follicular Fluid of Women with Endometriosis

Nahid Nasiri  1 , Ashraf Moini  2 , Poopak Eftekhari-Yazdi  1 , Leila Karimian  1 , Reza Salman-Yazdi  3 , Arezoo Arabipoor  4
Affiliations

Oxidative Stress Statues in Serum and Follicular Fluid of Women with Endometriosis

Nahid Nasiri et al. Cell J. 2017 Winter.

Abstract

Objective: This study aimed to evaluate the levels of two oxidative stress (OS) markers including lipid peroxide (LPO) and total antioxidant capacity (TAC) in both serum and follicular fluid (FF) of women with endometriosis after puncture.

Materials and methods: In this cross-sectional study, a total number of sixty-three women younger than 40 years old with laparoscopy (gold standard for endometriosis diagnosis) indication underwent in vitro fertilization (IVF) program in the Royan Institute, Tehran, Iran from September 2013 to October 2014. About forty-three patients were diagnosed with endometriosis after laparoscopy. Blood and FF from the leading follicle in each stimulated ovary were obtained at the time of egg retrieval; samples were centrifuged and frozen until assessment. At the time of sample assessment, serum and FF samples were evaluated for the levels of LPO and TAC on spectrophotometery.

Results: We observed that women with endometriosis had significantly higher LPO and lower TAC levels in the serum and FF as compared with the control group (P<0.05).

Conclusion: It has observed that FF of women with endometriosis, regardless of disease stage, increases the proliferation power of endometrial cells in vitro, we presume that inflammatory reactions-induced OS in ovary may be responsible for proliferation induction ability in FF obtained from women with endometriosis.

Keywords: Endometriosis; Follicular Fluid; Lipid Peroxide; Oxidative Stress.

PubMed Disclaimer

Figures

Fig.1
Fig.1
TAC concentration in serum and FF of endometriosis and control women. Data are presented as mean ± SEM. Statistical significance level is P<0.05. TAC; Total antioxidant capacity and FF; Follicular fluid.
Fig.2
Fig.2
LPO concentration in serum and FF of endometriosis and control women. Data are presented as mean ± SEM. Statistical significance level is P<0.05. FF; Follicular fluid and LPO; Lipid peroxide.
See this image and copyright information in PMC

References

    1. Giudice LC, Kaol LC. Endometriosis. Lancet. 2004;364(9447):1789–1799. - PubMed
    1. Gupta S, Agarwal A, Krajcir N, Alvarez JG. Role of oxidative stress in endometriosis. Reprod Biomed Online. 2006;13(1):126–134. - PubMed
    1. Ngô C, Chéreau C, Nicco C, Weill B, Chapron C, Batteux F. Reactive oxygen species controls endometriosis progression. Am J Pathol. 2009;175(1):225–234. - PMC - PubMed
    1. Gupta S, Agarwal A, Sekhon L, Krajcir N, Cocuzza M, Falcone T. Serum and peritoneal abnormalities in endometriosis: potential use as diagnostic markers. Minerva Ginecol. 2006;58(6):527–551. - PubMed
    1. Healy DL, Rogers PA, Hii L, Wingfeild M. Angiogenesis: a new theory for endometriosis. Hum Reprod Update. 1998;4(5):736–740. - PubMed

LinkOut - more resources