Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016:2016:8460291.
doi: 10.1155/2016/8460291. Epub 2016 Nov 30.

Effect of Melatonin and Calmodulin in an Idiopathic Scoliosis Model

Jun-Zhe Wu  1 , Wen-Hua Wu  1 , Li-Jiang He  1 , Qing-Feng Ke  1 , Long Huang  1 , Zhang-Sheng Dai  1 , Yu Chen  2
Affiliations

Effect of Melatonin and Calmodulin in an Idiopathic Scoliosis Model

Jun-Zhe Wu et al. Biomed Res Int. 2016.

Abstract

Background. To explore influence of continuous illumination, luzindole, and Tamoxifen on incidence of scoliosis model of rats. Methods. Thirty-two one-month-old female rats were rendered into bipedal rats. The bipedal rats were divided into 4 groups: group A by intraperitoneal injection of luzindole and continuous illumination; group B by intraperitoneal injection of luzindole only; group C by intraperitoneal injection of luzindole and oral administration of Tamoxifen; and group D by intraperitoneal injection of equivalent saline. Radiographs were taken at 8th week and 16th week, and incidence and the Cobb angles of scoliosis were calculated. At 16th week, all rats were sacrificed. Before the sacrifice, the levels of calmodulin were measured in each group. Results. At 8th week, scoliosis occurred in groups A and B, with an incidence of 75% and 12.5%, respectively, while rats in group C or D had no scoliosis. At 16th week, scoliosis incidences in groups A and B were 57% and 62.5%, respectively. No scoliosis occurred in group C or D. Calmodulin in platelets in group B was significantly different, compared with groups A and D. There was no significant difference in calmodulin in platelets in groups B and C. Conclusion. By intraperitoneal injection of luzindole in bipedal rats, scoliosis rat models could be successfully made. Under light, incidence of scoliosis may be increased at an early period but it is reversible. Tamoxifen can suppress natural process of scoliosis.

PubMed Disclaimer

Conflict of interest statement

All authors have no conflict of interests in this study.

Figures

Figure 1
Figure 1
Radiograph taken for the bipedal rat in each group at 8th week.
Figure 2
Figure 2
Radiograph taken for the bipedal rat in each group at 16th week.
See this image and copyright information in PMC

References

    1. Dai J., Inscho E. W., Yuan L., Hill S. M. Modulation of intracellular calcium and calmodulin by melatonin in MCF-7 human breast cancer cells. Journal of Pineal Research. 2002;32(2):112–119. doi: 10.1034/j.1600-079x.2002.1844.x. - DOI - PubMed
    1. Machida M., Dubousset J., Imamura Y., et al. A possible role in pathogenesis of adolescent idiopathic scoliosis. Spine. 1996;21(10):1147–1152. - PubMed
    1. Ma X. S., Lv F. Z., Wu J. Z., et al. Making models of bipedal rat scoliosis by luzindole. Chinese Journal of Spine and Spinal Cord. 2009;10:774–777.
    1. Benítez-King G., Huerto-Delgadillo L., Antón-Tay F. Melatonin modifies calmodulin cell levels in MDCK and N1E-115 cell lines and inhibits phosphodiesterase activity in vitro. Brain Research. 1991;557(1-2):289–292. doi: 10.1016/0006-8993(91)90146-M. - DOI - PubMed
    1. Antón-Tay F., Martínez I., Tovar R., Benítez-King G. Modulation of the subcellular distribution of calmodulin by melatonin in MDCK cells. Journal of Pineal Research. 1998;24(1):35–42. doi: 10.1111/j.1600-079X.1998.tb00363.x. - DOI - PubMed

LinkOut - more resources