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. 2017 Jan 3;12(1):e0168828.
doi: 10.1371/journal.pone.0168828. eCollection 2017.

The rs7044343 Polymorphism of the Interleukin 33 Gene Is Associated with Decreased Risk of Developing Premature Coronary Artery Disease and Central Obesity, and Could Be Involved in Regulating the Production of IL-33

Javier Angeles-Martínez  1 , Rosalinda Posadas-Sánchez  2 , Luis Llorente  3 , Edith Alvarez-León  1 , Julian Ramírez-Bello  4 , Teresa Villarreal-Molina  5 , Guadalupe Lima  3 , Guillermo Cardoso-Saldaña  2 , José Manuel Rodríguez-Pérez  1 , Nonanzit Pérez-Hernández  1 , José Manuel Fragoso  1 , Carlos Posadas-Romero  2 , Gilberto Vargas-Alarcón  1
Affiliations

The rs7044343 Polymorphism of the Interleukin 33 Gene Is Associated with Decreased Risk of Developing Premature Coronary Artery Disease and Central Obesity, and Could Be Involved in Regulating the Production of IL-33

Javier Angeles-Martínez et al. PLoS One. .

Abstract

Aim: The effect of interleukin 33 (IL-33) in the inflammatory process generates significant interest in the potential significance of IL-33 as a biomarker for coronary artery disease (CAD). Here, our objective was to analyze whether IL-33 gene polymorphisms are associated with premature CAD in a case-control association study.

Methods: Four IL-33 polymorphisms (rs7848215, rs16924144, rs16924159 and rs7044343) were genotyped by 5' exonuclease TaqMan assays in 1095 patients with premature CAD and 1118 controls.

Results: The rs7044343 T allele was significantly associated with a diminished risk of premature CAD (OR = 0.81, 95% CI: 0.69-0.97, Pdom = 0.020; OR = 0.85, 95% CI: 0.75-0.96, Padd = 0.019) and central obesity (OR = 0.74, 95% CI: 0.58-0.93, Pdom = 0.0007), respectively. When patients were divided into groups with and without type 2 diabetes mellitus (T2DM), the rs7044343 T allele was associated with a reduced risk of premature CAD in patients without (OR = 0.85, 95% CI: 0.73-0.99, Padd = 0.038) and with T2DM (OR = 0.61, 95% CI: 0.38-0.97, Pdom = 0.039; OR = 0.69, 95% CI: 0.49-0.97, Padd = 0.035). In order to establish the functional effect of the rs7044343 polymorphism, the production of IL-33 was determined in monocytes of selected individuals. Monocytes from individuals with rs7044343 CC genotype produced higher levels of IL-33 than monocytes from individuals with other genotypes.

Conclusion: The results suggest that the IL-33 rs7044343 T allele could be a susceptibility marker for premature CAD and central obesity. The rs7044343 polymorphism could be involved in regulating the production of IL-33.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Detection of IL-33 in monocytes from healthy individuals with rs7044343 CC, TC and TT genotypes (21 participants were included for each genotype).
Data are presented as mean ± SD. CC vs CT (P = 0.005) and CC vs TT (P = 0.007).
See this image and copyright information in PMC

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