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. 2017 Jan 3;12(1):e0169213.
doi: 10.1371/journal.pone.0169213. eCollection 2017.

Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging

Augusto Schneider  1   2 , Scot J Matkovich  3 , Berta Victoria  2 , Lina Spinel  2 , Andrzej Bartke  4 , Pawel Golusinski  2   5   6 , Michal M Masternak  2   6
Affiliations

Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging

Augusto Schneider et al. PLoS One. .

Abstract

The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 months of age for miRNA sequencing. We detected a total of 404 miRNAs in the ovarian samples, from which the abundance of 22 and 33 miRNAs changed with age in N and df/df mice, respectively. Of these, only three miRNAs were commonly regulated with age between N and df/df mice, indicating a very divergent miRNA profile between genotypes. We also detected that 46 miRNAs were regulated between N and df/df mice, of which 23 were regulated exclusively in young mice, 12 exclusively in old mice and 12 commonly regulated at young and old ages. Many genes likely to be targeted by these miRNAs are involved in the FoxO, mTOR, PI3k/Akt and insulin signaling pathways. These results suggest that the aging process has a differential impact on the ovarian miRNA profile in df/df mice, and suggest that these miRNAs can be central players in the maintenance of a younger ovarian phenotype.

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Conflict of interest statement

The authors disclose no competing interests.

Figures

Fig 1
Fig 1. Unsupervised hierarchical clustering ordered by the adjusted level of miRNA expression for the top 50 most expressed miRNAs in ovaries of df/df (n = 10; Young–DY and Old–DO) and N mice (n = 10; Young–NY and Old–NO).
Fig 2
Fig 2. Abundance of the top 50 most expressed miRNAs in ovarian samples.
The values are an average reads per million (rpm) from Ames dwarf (df/df) and Normal (N) mice at 6 and 22 months of age.
Fig 3
Fig 3
Venn diagram indicating individual miRNAs (A) and miRNAs gene families (B), based on miRBase classification of highly similar miRNAs, that were significantly regulated between Ames dwarf (df/df) mice with aging, and between young (6 months old) and aged (22 months old) df/df and Normal (N) mice.
Fig 4
Fig 4. Schematic representation of the main target genes of the microRNAs differentially regulated between Normal (N) and Ames dwarf (df/df) mice at both ages (6 and 22 months).
Green–target gene of a regulated miRNA; Blue–non regulated target gene.
See this image and copyright information in PMC

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