Comparison of fecal microbiota of three captive carnivore species inhabiting Korea

Abstract

This study aimed at characterizing fecal microbiota of three captive carnivore species of leopard cats Prionailurus bengalensis, Eurasian otters Lutra lutra and raccoon dogs Nyctereutes procyonoides. We used DNA barcoding sequencing to analyze 16S rRNA genes of uncultured bacteria in the feces collected in the Seoul Zoo. The sequencing analyses revealed that: 1) Firmicutes was the most dominant phylum for all three animals; 2) bacterial genus-rank compositions were distinct across species of the animals; and 3) bacterial community memberships were different across species of the studied animals. We expect such baseline information is useful for better understanding of these endangered species and future management of their health in zoos.

Fig. 1.

Relative abundances of bacterial phyla. The numbers in the sample names indicate sampling days (YYMMDD). The alphabets indicate L for leopard cats, O for otters and R for raccoon dogs.

Fig. 2.

Relative abundances of the 30 most abundant taxa. The numbers in the sample names indicate sampling days (YYMMDD). The alphabets indicate L for leopard cat, O for otter and R for raccoon dog.

Fig. 3.

Principal coordinates analysis plots of the Jaccard and Yue and Clayton distances based on operational taxonomic units (OTUs) at 97% sequence identity. The libraries with less than 1,200 sequences were not included for the analyses.