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. 2016 Nov;10(11):ZC01-ZC04.
doi: 10.7860/JCDR/2016/18329.8762. Epub 2016 Nov 1.

Anti-microbial Efficacy of Soursop Leaf Extract (Annona muricata) on Oral Pathogens: An In-vitro Study

Bh Mithun Pai  1 , Gururagavendra Rajesh  2 , Ramya Shenoy  1 , Ashwini Rao  3
Affiliations

Anti-microbial Efficacy of Soursop Leaf Extract (Annona muricata) on Oral Pathogens: An In-vitro Study

Bh Mithun Pai et al. J Clin Diagn Res. 2016 Nov.

Abstract

Introduction: Annona muricata also called as Soursop is a, flowering evergreen tree native to Mexico, Cuba, Central America and parts of India. The miracle tree as it is widely known as a natural cancer killer that is 10,000 times stronger than chemotherapy. Based on these miraculous claims, the leaves of these plants were used as an extract at varying concentrations as an antibacterial agent against oral pathogens.

Aim: The aim of the study was to assess antimicrobial efficacy of Soursop leaf extarct (Annona muricata) on Streptococcus mutans, Streptococcus mitis, Porphyromonas gingivalis, Prevotella intermedia and Candida albicans using disc diffusion method.

Materials and methods: Extracts of Annona muricata leaves of concentrations of 1%, 5%, 10%, 15% and 20% were prepared. The anti-microbial efficacy was evaluated using disc diffusion method against Streptococcus mutans, Streptococcus mitis, Porphyromonas gingivalis, Prevotella intermedia and Candida albicans on agar plates.

Results: All concentrations of extracts were effective on the microbiota except for the P. Intermedia. The Soursop extract was highly effective on Candida species, with all concentrations exhibiting bactericidal and fungicidal property. The extracts at different concentration were effective when compared to the gold standard controls and the effect was statistically significant (p<0.05). Data obtained was analysed using one way analysis of variance (ANOVA) and Tukey's post-hoc test.

Conclusion: The Soursop extracts were efficient for all test organisms expect P. intermedia. The present study demonstrated the in-vitro efficacy of Soursop was highest against S. mutans followed by C. albicans and least on P. intermedia. Hence, this study proves to an extent that the Soursop extract when used against oral microbiota has sufficient anti-microbial and fungicidal property.

Keywords: Dental caries; Periodontal disease; Streptococcus mutans.

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Figures

[Table/Fig-2]:
[Table/Fig-2]:
Mean efficacy values of Soursop extracts, respective Gold standard and positive control (Chlorhexidine 0.2%) on oral pathogens.
See this image and copyright information in PMC

References

    1. Phillipson JD. Phytochemistry and medicinal plants. Phytochemistry. 2001;56:237–43. - PubMed
    1. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109:69–75. - PMC - PubMed
    1. Mehta VV, Rajesh G, Rao A, Shenoy R, Pai BHM. Antimicrobial efficacy of punica granatum mesocarp, nelumbo nucifera leaf, psidium guajava leaf and coffea canephora extract on common oral pathogens: an in-vitro study. J Clin Diagn Res. 2014;8:65–68. - PMC - PubMed
    1. Hamizah S, Roslida AH, Fezah O, Tan KL, Tor YS, Tan CI. Chemopreventive potential of annona muricata l leaves on chemically-induced skin papillomagenesis in mice. Asian Pacific J Cancer Prev. 2012;13(6):2533–39. - PubMed
    1. Facey PC, Pascoe KO, Porter RB, Jones AD. Investigation of plants used in Jamaican folk medicine for anti-bacterial activity. J Pharm Pharmacol. 1999;51(120):1455–60. - PubMed

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