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. 2017 Dec;7(1):2.
doi: 10.1186/s13568-016-0301-1. Epub 2017 Jan 3.

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

Banoth Srinivas  1 , Ganapathiwar Swarupa Rani  1 , Bhukya Kiran Kumar  1 , Banoth Chandrasekhar  1 , Kommalapati Vamsi Krishna  2 , Tangutur Anjana Devi  2 , Bhukya Bhima  3
Affiliations

Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm

Banoth Srinivas et al. AMB Express. 2017 Dec.

Abstract

The purpose of this study is to evaluate the probiotic characteristics of 15 yeast strains isolated from nectar of toddy palm. Initially, the collected samples were inoculated on yeast extract peptone dextrose agar plates and the colonies so obtained were culturally and morphologically characterized. Commercial probiotic yeast, Saccharomyces boulardii served as the control in these experiments. Of the 15 yeast strains, the isolates that were resistant to antibiotics and worked synergistically with other cultures were considered for further evaluation. Selected isolates were evaluated in vitro for tolerance to simulated gastrointestinal conditions such as temperature, pH, bile and gastric juice. Further the yeast isolates were evaluated for their pathogenicity and adherence to intestinal epithelial cells. The 2 yeast isolates with efficient probiotic properties were finally characterized by sequencing their 5.8 S rRNA and partial sequences of internal transcribed spacer 1 and 2. The sequences were BLAST searched in the National Center for Biotechnology Information, nucleic acid database for sequence similarity of organisms and phylogenetic evolutionary analysis was carried out. Based on maximum similarity of basic local alignment search tool results, organisms were characterized as Pichia kudriavzevii OBS1 (100%) and Saccharomyces cerevisiae OBS2 (96%) and sequences were finally deposited in the GenBank data library. Among these two isolates, S. cerevisiae OBS2 displayed slight/moderate antioxidant and anticancer property. Hence, strain OBS2 can be utilized and explored as a potential probiotic for therapeutic applications.

Keywords: Antimicrobial resistance; Probiotics; Therapeutics; Toddy nectar; Yeasts.

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Figures

Fig. 1
Fig. 1
Antimicrobial drug sensitivity of a yeast isolate OBS2 and b OBS1
Fig. 2
Fig. 2
Antagonistic activity of yeast isolates 1 OBS1, 2 OBS2, 3 OBS3, 4 OBS4, 5 OBS7, 6 OBS9, 7 OBS12 and 8 S. boulardii on human intestinal pathogens a Escherichia coli 0157:H7, b Pseudomonas aeruginosa, c Klebsiella pneumonia, d Staphylococcus aureus, e Salmonella typhi and f Salmonella paratyphi
Fig. 3
Fig. 3
Pathogenicity of yeast isolates and commercial probiotic S. boulardii: a protease activity, b phospholipase activity and c haemolytic activity
Fig. 4
Fig. 4
Pathogenicity of yeast isolates based on gene amplification: a genomic DNA of the yeast isolates and b amplicons of yeast isolates
Fig. 5
Fig. 5
Phylogenetic dendrogram of yeast isolates a P. kudriavzevii OBS1 and b S. cerevisiae OBS2
Fig. 6
Fig. 6
Effect of yeast isolates on viability of a breast cancer cell line MCF7 and b neuroblastoma cell line IMR32
See this image and copyright information in PMC

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