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. 2016 Dec;7(6):356-359.
doi: 10.1016/j.phrp.2016.10.004. Epub 2016 Oct 21.

No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population

Jeong Eun Lee  1 , Yong-Moon Lee  2 , In-Ock Seong  2 , Min-Woong Kang  3 , Choong Sik Lee  2 , Kyung-Hee Kim  2
Affiliations

No Detection of Episomal or Integrated High-Risk Human Papillomavirus in Nonsmall Cell Lung Carcinomas among Korean Population

Jeong Eun Lee et al. Osong Public Health Res Perspect. 2016 Dec.

Abstract

Objectives: High-risk human papillomavirus (hrHPV) is known to be a representative cancer-causing agent in the genital and head and neck regions. Many studies have detected hrHPV DNA in nonsmall cell lung carcinoma. However, hrHPV-etiologic correlation in nonsmall cell lung carcinoma remains unclear. This study is designed to determine the prevalence of episomal or integrated hrHPV DNA in nonsmall cell lung carcinoma among the Korean population.

Methods: Surgically resected nonsmall cell lung carcinoma tissues, including 134 cases of squamous cell carcinoma (SqCC) and 99 cases of adenocarcinoma (ADC), were examined. In situ hybridization (ISH) for detecting episomal or integrated hrHPV DNA was performed using the INFORM HPV III Family 16 Probe (B) in the Ventana-validated assay. Anyplex II HPV28 detection kit based on real-time polymerase chain reaction was used for HPV DNA detection and genotyping.

Results: All members of the study population were of Korean ethnicity. Episomal or integrated hrHPV DNA ISH analysis result was negative in all 233 cases. One SqCC of 89 samples (42 SqCCs and 47 ADCs) was positive for an hrHPV genotype by Anyplex II HPV28 detection kit.

Conclusion: Our finding did not demonstrate hrHPV-etiologic correlation in primary lung SqCC and ADC in the Korean population.

Keywords: adenocarcinoma; human papillomavirus; in situ hybridization; lung; squamous cell carcinoma.

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Figures

Figure 1
Figure 1
Squamous cell carcinoma. (A) A laryngeal squamous cell carcinoma positive for high-risk human papillomavirus (hrHPV). (B) A lung squamous cell carcinoma-negative in situ hybridization for hrHPV (Scale bar: 60 μm).
See this image and copyright information in PMC

References

    1. Marur S., D'Souza G., Westra W.H. HPV-associated head and neck cancer: a virus-related cancer epidemic. Lancet Oncol. 2010 Aug;11(8):781–789. - PMC - PubMed
    1. Schiffman M., Castle P.E., Jeronimo J. Human papillomavirus and cervical cancer. Lancet. 2007 Sep 8;370(9590):890–907. - PubMed
    1. Parkin D.M., Bray F. Chapter 2: the burden of HPV-related cancers. Vaccine. 2006 Aug 31;24(Suppl 3) S3/11–25. - PubMed
    1. Bishop J.A., Ma X.J., Wang H. Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6/E7 mRNA in situ hybridization method. Am J Surg Pathol. 2012 Dec;36(12):1874–1882. - PMC - PubMed
    1. Bucchi D., Stracci F., Buonora N. Human papillomavirus and gastrointestinal cancer: a review. World J Gastroenterol. 2016 Sep 7;22(33):7415–7430. - PMC - PubMed