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. 2016 Dec 21;22(47):10364-10370.
doi: 10.3748/wjg.v22.i47.10364.

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Wei-Zhong Sheng  1 , Yu-Sheng Chen  1 , Chuan-Tao Tu  1 , Juan He  1 , Bo Zhang  1 , Wei-Dong Gao  1
Affiliations

ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Wei-Zhong Sheng et al. World J Gastroenterol. .

Abstract

Aim: To explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer.

Methods: Western blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells.

Results: Compared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells.

Conclusion: ANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer.

Keywords: Angiopoietin-like protein 2; Cell invasion; Cell proliferation; Gastric cancer.

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Conflict of interest statement

Conflict-of-interest statement: We declare that there are no conflicts of interest to disclose.

Figures

Figure 1
Figure 1
Western blot detected the protein expression of ANGPTL2 in gastric cell lines. ANGPTL2: Angiopoietin-like protein 2.
Figure 2
Figure 2
Stable overexpression of ANGPTL2 contributed to proliferation of gastric cancer cells. aP < 0.05 vs the control group and Lv-NC group. ANGPTL2: Angiopoietin-like protein 2.
Figure 3
Figure 3
Knockdown of ANGPTL2 weakened the proliferation of gastric cancer cells. aP < 0.05 vs the control group and Lv-NC-shRNA group. ANGPTL2: Angiopoietin-like protein 2.
See this image and copyright information in PMC

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