Immunohistochemical localization of a beta-galactoside-binding lectin in rat central nervous system. II. Light- and electron-microscopical studies in developing cerebellum

Abstract

An endogenous brain lectin exhibiting beta-galactoside specificity (RBL-16) was localized during postnatal cerebellum development both at the light- and electron-microscopical level. The lectin was widely distributed in neurons, astroglial and perivascular cells. Its levels were nearly constant during development in the two latter cell types. The lectin was developmentally regulated with a transient accumulation in Purkinje dendritic spines between the 10th- and 13th day, then it decreased until adult age. From electron-microscopical observations, it could be concluded that, in Purkinje cells, the lectin remained in the intracellular compartment, in dendrites and cell bodies. It was never externalized in the region where synaptogenesis takes place. A role in the intracellular transport of molecules should be expected from such a localization. The lectin was also transiently found on the surface of postmitotic neuroblasts in the external germinative layer and on the parallel fibers of the upper part of the molecular layer. However, it was not expressed inside neuroblasts. This suggests that part of the lectin found on the surface of neuroblasts originates from heavily stained astrocytes which could secrete it. RBL-16 could be making bridges between neuroblasts in the premigratory zone and between growing axons. A role in transient neuroblast adhesion in the external germinative layer and in parallel fiber fasciculation is expected from such a localization.