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. 2016 Dec 21:11:87-88.
doi: 10.1016/j.gdata.2016.12.007. eCollection 2017 Mar.

Metagenomic analysis of microbial community of a parasitoid wasp Megaphragma amalphitanum

A V Nedoluzhko  1 , F S Sharko  2 , S V Tsygankova  1 , E S Boulygina  1 , A S Sokolov  2 , S M Rastorguev  1 , V V Kadnikov  2 , A V Mardanov  2 , N V Ravin  3 , A M Mazur  2 , A A Polilov  4 , N M Gruzdeva  1 , E B Prokhortchouk  3 , K G Skryabin  5
Affiliations

Metagenomic analysis of microbial community of a parasitoid wasp Megaphragma amalphitanum

A V Nedoluzhko et al. Genom Data. .

Abstract

The vast majority of multicellular organisms coexist with bacterial symbionts that may play various roles during their life cycle. Parasitoid wasp Megaphragma amalphitanum (Hymenoptera: Trichogrammatidae) belongs to the smallest known insects whose size is comparable with some bacteria. Using 16S rRNA gene sequencing and Whole Genome Sequencing (WGS), we described microbiota diversity for this arthropod and its potential impact on their lifecycle. Metagenomic sequences were deposited to SRA database which is available at NCBI with accession number SRX2363723 and SRX2363724. We found that small body size and limited lifespan do not lead to a significant reduction of bacterial symbionts diversity. At the same time, we show here a specific feature of microbiota composition in M. amalphitanum - the absence of the Rickettsiaceae family representatives that are known to cause sex-ratio distortion in arthropods and well represented in other populations of parasitoid wasps.

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Figures

Fig. 1
Fig. 1
Relative abundance of major bacterial OTUs associated with M. amalphitanum based on 16 rRNA fragment from pyrosequencing.
See this image and copyright information in PMC

References

    1. Sogin M.L., Morrison H.G., Huber J.A., Welch M.D., Huse S.M., Neal P.R., Arrieta J.M., Herndl G.J. Microbial diversity in the deep sea and the underexplored “rare biosphere”. Proc. Natl. Acad. Sci. U. S. A. 2006;103:12115–12120. - PMC - PubMed
    1. Lane D.J. Wiley; Chichester: 1991. 16S/23S rRNA Sequencing, nucleic acid techniques in bacterial systematics; pp. 115–175.
    1. Caporaso J.G., Kuczynski J., Stombaugh J., Bittinger K., Bushman F.D., Costello E.K., Fierer N., Peña A.G., Goodrich J.K., Gordon J.I., Huttley G.A., Kelley S.T., Knights D., Koenig J.E., Ley R.E., Lozupone C.A., McDonald D., Muegge B.D., Pirrung M., Reeder J., Sevinsky J.R., Turnbaugh P.J., Walters W.A., Widmann J., Yatsunenko T., Zaneveld J., Knight R. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods. 2010;7:335–336. - PMC - PubMed
    1. Edgar R.C. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010;26:2460–2461. - PubMed
    1. Wang Q., Garrity G.M., Tiedje J.M., Cole J.R. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl. Environ. Microbiol. 2007;73:5261–5267. - PMC - PubMed

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