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. 2017 Feb;14(2):141-144.
doi: 10.1038/nmeth.4141. Epub 2017 Jan 9.

Cryogenic optical localization provides 3D protein structure data with Angstrom resolution

Siegfried Weisenburger  1   2 , Daniel Boening  1 , Benjamin Schomburg  3 , Karin Giller  3 , Stefan Becker  3 , Christian Griesinger  3 , Vahid Sandoghdar  1   2
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Cryogenic optical localization provides 3D protein structure data with Angstrom resolution

Siegfried Weisenburger et al. Nat Methods. 2017 Feb.

Abstract

We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.

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