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. 2017 Jan 7:6:4.
doi: 10.1186/s13756-016-0163-y. eCollection 2017.

Shiga (Vero)-toxin producing Escherichia coli isolated from the hospital foods; virulence factors, o-serogroups and antimicrobial resistance properties

Reza Ranjbar  1 , Mojtaba Masoudimanesh  2 , Farhad Safarpoor Dehkordi  3 , Nematollah Jonaidi-Jafari  4 , Ebrahim Rahimi  5
Affiliations

Shiga (Vero)-toxin producing Escherichia coli isolated from the hospital foods; virulence factors, o-serogroups and antimicrobial resistance properties

Reza Ranjbar et al. Antimicrob Resist Infect Control. .

Abstract

Background: According to the presence of the weak, diabetic and immunosuppressive patients in hospitals, hospital foods should have a high quality and safety. Cooking a lot of foods higher than daily requirement, storage of cooked foods in an inappropriate condition and presence of nurses and servants in distribution of food to patients are the main reasons caused contamination of hospital foods. Shiga toxigenic Escherichia coli is one of the common cause of food poisoning in hospitals. The present research was carried out to study the distribution of virulence factors, O-serogroups and antibiotic resistance properties in STEC strains recovered from Iranian hospital food samples.

Methods: Five-hundred and eighty raw and cooked food samples were collected and immediately transferred to the laboratory. E. coli-positive strains were subjected to PCR and disk diffusion method.

Results: Thirty-nine out of 580 (6.72%) hospital food samples were contaminated with E. coli. Raw (20%) and cooked meat (6%) were the most commonly contaminated samples. Raw samples had the higher prevalence of E. coli (P <0.01). Samples which were collected in the summer season had the highest prevalence of bacteria (64.10%). Significant difference was seen between the prevalence of EHEC and AEEC subtypes (P <0.01). The most commonly detected virulence factors in both EHEC and AEEC subtypes were stx1 and eae. The most commonly detected serogroups were O26 (43.75%) and O157 (25%) and there were no positive results for O103, O145, O91, O113 and O128 serogroups. Aac (3)-IV (100%), CITM (100%) and tetA (62.50%) were the most commonly detected antibiotic resistance genes. STEC strains harbored the highest levels of resistance against ampicillin (93.75%), gentamycin (93.75%), tetracycline (87.50%) and ciprofloxacin (81.25%). All of the STEC strains were resistant to at least 3 antibiotics, while the prevalence of resistance against more than 12 antibiotics were 12.50%.

Conclusions: High presence of O157 serogroups, EHEC strains and animal-based antibiotics in cooked foods showed insufficiency of cooking time and temperature in the kitchens of hospitals. Judicious prescription of antibiotics and attentions to the principles of food safety can reduce the risk of resistant and virulent strains of STEC in hospital foods.

Keywords: Antibiotic resistance properties; Hospital foods; Iran; O-serogroups; Shiga-toxin producing escherichia coli; Virulence factors.

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Figures

Fig. 1
Fig. 1
Seasonal distribution of Escherichia coli in the hospital food samples
Fig. 2
Fig. 2
Prevalence of multi-drug resistant strains of Shiga toxigenic Escherichia coli isolated from hospital food samples
See this image and copyright information in PMC

References

    1. Gould LH, Walsh KA, Vieira AR, Herman K, Williams IT, Hall AJ, Cole D. Surveillance for foodborne disease outbreaks—United States, 1998–2008. MMWR Surveill Summ. 2013;62:1–34. - PubMed
    1. Havelaar AH, Kirk MD, Torgerson PR, Gibb HJ, Hald T, Lake RJ, Praet N, Bellinger DC, De Silva NR, Gargouri N. World Health Organization Global estimates and regional comparisons of the burden of foodborne disease in 2010. PLoS Med. 2015;12:e1001923. doi: 10.1371/journal.pmed.1001923. - DOI - PMC - PubMed
    1. Control CD. Prevention. Surveillance for Foodborne Disease Outbreaks, United States, 2013, Annual Report. Atlanta: US Department of Health and Human Services; 2014.
    1. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson M-A, Roy SL, Jones JL, Griffin PM. Foodborne illness acquired in the United States—major pathogens. Emerg Infect Dis. 2011;17:7–15. doi: 10.3201/eid1701.P11101. - DOI - PMC - PubMed
    1. Stewardson AJ, Renzi G, Maury N, Vaudaux C, Brassier C, Fritsch E, Pittet D, Heck M, van der Zwaluw K, Reuland EA. Extended-Spectrum β-Lactamase–Producing Enterobacteriaceae in Hospital Food: A Risk Assessment. Infect Control Hosp Epidemiol. 2014;35:375–83. doi: 10.1086/675609. - DOI - PubMed

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