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. 2016 Nov-Dec;24(6):575-581.
doi: 10.1590/1678-775720150553.

Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Marcela Paola Castro Arias  1 , Amanda Garcia Alves Maliza  1 , Raquel Zanin Midena  1 , Márcia Sirlene Zardin Graeff  2 , Marco Antonio Húngaro Duarte  1 , Flaviana Bombarda de Andrade  1
Affiliations

Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Marcela Paola Castro Arias et al. J Appl Oral Sci. 2016 Nov-Dec.

Abstract

Objective: The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture.

Material and methods: Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye.

Results: The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05).

Conclusion: The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules.

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Figures

Figure 1
Figure 1. CLSM images after medication for 15 d. A: calcium hydroxide group. B: calcium hydroxide with ultrasonic agitation. C: positive control after contamination protocol without medication. Bars: 20.0 µm. Stained bacteria inside tubules are green or red, which means alive or dead, respectively
Figure 2
Figure 2. Graphics of median and min-max values of bacterial viability. A: Global comparison of the two groups after intracanal medication, with ultrasonic agitation (CHU) or without (CH). B: Bacterial viability in different thirds, with or without ultrasound, cervical (CHC and CHUC) and medium (CHM and CHUM). C: Bacterial viability in different depths, with or without ultrasound, superficial (CH sup and CHU sup) or deep (CH deep and CHU deep)
Figure 3
Figure 3. Amount of CFU/mL per group after collection with Largo burs #5 and #6; amount of total CFU/mL and amount of plates that did not present bacterial growth
Figure 4
Figure 4. Comparison of scores of intracanal medication penetration inside tubules of the two groups, calcium hydroxide (CH) and calcium hydroxide with ultrasonic agitation (CHU). A: Comparison of penetrability in different thirds of the root canal, apical (CHA, CHUA), medium (CHM, CHUM), and cervical (CHC, CHUC). B: Comparison of penetrability in different depths of dentinal mass, superficial (CH sup and CHU sup) or deep (CH deep and CHU deep)
Figure 5
Figure 5. Images by CLSM after penetration of medication stained with 1% rodamin. A and B (HC): calcium hydroxide group. C and D: calcium hydroxide with ultrasonic agitation (HCuS). Bars: 20.0 µm
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References

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