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. 2017:1557:207-212.
doi: 10.1007/978-1-4939-6780-3_19.

Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L

Fatima Bilal  1   2   3 , Michaël Pérès  1   2   4 , Nathalie Andrieu-Abadie  1   2 , Thierry Levade  1   2   4   5 , Bassam Badran  3 , Ahmad Daher  3 , Bruno Ségui  6   7   8
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Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L

Fatima Bilal et al. Methods Mol Biol. 2017.

Abstract

Sphingomyelin synthases 1 and 2 convert the anti-oncometabolite ceramide to sphingomyelin, the most abundant sphingolipid in plasma membrane. CD95L-induced ceramide increase is associated with the caspase-dependent inhibition of sphingomyelin synthesis, which enhances the mitochondrial route to apoptosis. Knocking down sphingomyelin synthase 1 or inhibiting sphingomyelin synthesis facilitates ceramide accumulation, cytochrome c release from mitochondria, and caspase-9 activation in cancer cell upon CD95L treatment. Here, we describe a method to monitor in situ sphingomyelin synthase activity changes triggered by CD95L.

Keywords: Apoptosis; Ceramide; In situ sphingomyelin synthase activity; Sphingomyelin.

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