Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Apr;39(4):607-612.
doi: 10.1007/s10529-017-2285-6. Epub 2017 Jan 16.

Transient expression of recombinant tissue plasminogen activator (rt-PA) gene in cucurbit plants using viral vector

Vahid Jalali Javaran  1 , Alireza Shafeinia  1 , Mokhtar Jalali Javaran  2 , Esmaeil Ghasemi Gojani  1 , Malihe Mirzaee  3
Affiliations

Transient expression of recombinant tissue plasminogen activator (rt-PA) gene in cucurbit plants using viral vector

Vahid Jalali Javaran et al. Biotechnol Lett. 2017 Apr.

Abstract

Objective: To use a transient expression system to express a truncated human tissue plasminogen activator (K2S) gene in cucurbit plants.

Results: The recombinant tissue plasminogen activator protein (K2S form) was expressed in active form in cucurbit plants. Its molecular weight was 43 kDa. The plant-derived rt-PA was determined using goat anti-rabbit antibody by western blotting. Among the infected lines, the highest expression of rt-PA was 62 ng/100 mg per leaf tissue as measured by ELISA. The enzymatic activity of the plant-derived rt-PA was 0.8 IU/ml.

Conclusions: The K25 form of rt-PA was expressed for the first time using the viral expression system. Plant-derived rt-PA showed similar potency to commercially-available PA.

Keywords: Cucurbit plant; Human tissue plasminogen activator (K2S); Molecular pharming; Transient gene expression.

PubMed Disclaimer

MeSH terms

LinkOut - more resources