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. 2016:2016:9183861.
doi: 10.1155/2016/9183861. Epub 2016 Dec 20.

Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Haixiang Qiu  1 , Patrick John Kelly  2 , Jilei Zhang  1 , Qinghua Luo  1 , Yi Yang  1 , Yongjiang Mao  3 , Zhangping Yang  3 , Jing Li  1 , Hongzhuan Wu  4 , Chengming Wang  5
Affiliations

Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Haixiang Qiu et al. Can J Infect Dis Med Microbiol. 2016.

Abstract

Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for Anaplasma spp. and Ehrlichia spp. Positive samples were further analyzed with a standard PCR for the gltA. Anaplasma spp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29). Ehrlichia spp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing of gltA PCR products showed that A. marginale, A. ovis, Ehrlichia canis, and Ehrlichia sp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be A. platys, A. phagocytophilum, Anaplasma sp. BL126-13 (KJ410243), and Anaplasma sp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety of Anaplasma spp. and Ehrlichia spp.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Detection of Anaplasma spp. and Ehrlichia spp. in ruminants from 12 provinces of China. Blood samples of ruminants (2,240) were collected from twelve provinces (in bisque) of China. The prevalence is shown for Anaplasma spp. (red) and Ehrlichia spp. (blue).
Figure 2
Figure 2
Alignment of the sequences obtained with the 16S rRNA FRET-qPCR we used in our study and those of Anaplasma spp. in GenBank. “.” denotes the identical nucleotide sequence to that of A. bovis. Organisms with GenBank accession numbers identified in the study are in red.
Figure 3
Figure 3
Phylogenetic comparison of Anaplasma spp. from ruminants in this study. The 16S rRNA sequences (in red font and accession number) are compared with those of other representing Anaplasma spp. (in black font and accession number). Branch lengths are measured in nucleotide substitutions and numbers show branching percentages in bootstrap replicate. Scale bar represents the percent sequence diversity.
Figure 4
Figure 4
Phylogenetic comparison of Ehrlichia spp. from ruminants in this study. The gltA sequences (in red font and accession number) are compared with those of other representing Ehrlichia spp. (in black font and accession number). Branch lengths are measured in nucleotide substitutions and numbers show branching percentages in bootstrap replicate. Scale bar represents the percent sequence diversity.
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