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. 2017 Feb;23(2):304-307.
doi: 10.3201/eid2302.160649.

Nosocomial Infections with IMP-19-Producing Pseudomonas aeruginosa Linked to Contaminated Sinks, France

Lucie Amoureux, Karena Riedweg, Angélique Chapuis, Julien Bador, Eliane Siebor, André Péchinot, Marie-Lorraine Chrétien, Claire de Curraize, Catherine Neuwirth

Nosocomial Infections with IMP-19-Producing Pseudomonas aeruginosa Linked to Contaminated Sinks, France

Lucie Amoureux et al. Emerg Infect Dis. 2017 Feb.

Abstract

We isolated IMP-19-producing Pseudomonas aeruginosa from 7 patients with nosocomial infections linked to contaminated sinks in France. We showed that blaIMP-19 was located on various class 1 integrons among 8 species of gram-negative bacilli detected in sinks: P. aeruginosa, Achromobacter xylosoxidans, A. aegrifaciens, P. putida, Stenotrophomonas maltophilia, P. mendocina, Comamonas testosteroni, and Sphingomonas sp.

Keywords: France; IMP-19; Pseudomonas aeruginosa; antimicrobial resistance; bacteria; contaminated sinks; hospital; integrons; metallo-β-lactamases; nosocomial infections.

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Figures

Figure 1
Figure 1
Environmental testing for IMP-19 producers in investigation of nosocomial infections linked to contaminated sinks, France. A) Sink (blue circles), swab specimen of sink, and drain (red circles) tested. B) Flowchart of testing procedure.
Figure 2
Figure 2
Unweighted pair group method with arithmetic mean cluster analysis of XbaI-generated pulsotypes constructed with Dice coefficients for the 7 clinical isolates and the 7 environmental isolates of IMP-19−producing Pseudomonas aeruginosa linked to contaminated sinks, France. Isolates are indicated on dendrogram branches. The Dice coefficient scale is indicated at the bottom of the dendrogram.
Figure 3
Figure 3
Diversity of integrons harboring blaIMP-19 isolated from patients with nosocomial infections linked to contaminated sinks, France. Arrows indicate direction of transcription. a–f, suI-type class 1 integrons; g, Tn402−like class 1 integron. aac(6′)-Ib, aminoglycoside 6′-N-acetyltransferase; aadA13, aminoglycoside adenyltransferase ANT(3′); aadB, aminoglycoside-2′′-O-nucleotidyltransferase; blaIMP-19, metallo-β-lactamase IMP-19; fused qacG, aminoglycoside 6′-N-acetyltransferase; intl1, class 1 integron integrase; orf5, open reading frame 5; qacEΔ1, multidrug exporter; sul1, dihydropteroate synthase; tniQ, transposition protein; tniR, resolvase.
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