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. 2017 Mar:46:64-67.
doi: 10.1016/j.biologicals.2016.12.009. Epub 2017 Jan 15.

Virome of US bovine calf serum

Mohammadreza Sadeghi  1 , Beatrix Kapusinszky  2 , Danielle M Yugo  3 , Tung Gia Phan  2 , Xutao Deng  2 , Isis Kanevsky  4 , Tanja Opriessnig  5 , Amelia R Woolums  6 , David J Hurley  7 , Xiang-Jin Meng  3 , Eric Delwart  8
Affiliations

Virome of US bovine calf serum

Mohammadreza Sadeghi et al. Biologicals. 2017 Mar.

Abstract

Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.

Keywords: Bosavirus; Cell culture; Metagenomics; Parvovirus.

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Figures

Figure 1
Figure 1
Genome structure of novel parvovirus reported in this study. Maximum likelihood phylogenies are showing the relationship of the novel Bosavirus and BPV2 (A and B). Phylogenetic trees generated with NS and VP of Bosavirus and the representatives of ten genera in the subfamily Parvovirinae.
Figure 2
Figure 2
VP and NS amino acid sequences identities between Bosavirus and Copiparvoviruses. The lower-left triangle shows homology between VP1 protein sequences of the six Copiparvoviruses generated by ClustalW alignment using Geneious Pro 6.1.8 software with default settings. The upper-right triangle shows NS amino acid sequence identities against of the six Copiparvoviruses.
See this image and copyright information in PMC

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