Study on experimental model of coronary artery thrombogenesis and acute myocardial infarction: changes in epicardial ECG, platelet function and biochemistry, and pathology

Abstract

A model was produced for formation of coronary artery thrombosis and development of acute myocardial infarction (AMI) in experimental dogs (n = 8) by injuring endothelium of coronary artery with a steel wire, inserting a copper wire into the lumen, and producing local stenosis with a constrictor. The results showed that ST segment of epicardial ECG began to elevate 15-30 min after handling the coronary artery, platelets aggregation rate and plasma TXB2 increased rapidly, plasma 6-k-PGF1 alpha did not change, platelets cGMP rose continuously, while platelets cAMP rose only at 60 min after handling the coronary artery. Histological findings confirmed the occurrence of artery thrombus and myocardial necrosis. The model may be helpful in investigating the whole process of activation of platelet, thrombogenesis and development of AMI, with the changes in biochemistry, structure, pathophsiology and ECG monitored during the period.