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. 2016 Dec;5(6):665-666.
doi: 10.3892/br.2016.794. Epub 2016 Oct 27.

Detection of stanozolol in the urine of athletes at a pg level: The possibility of passive exposure

Christina Tsitsimpikou  1 , Konstantinos Tsarouhas  2 , Demetrios A Spandidos  3 , Aristides M Tsatsakis  4
Affiliations

Detection of stanozolol in the urine of athletes at a pg level: The possibility of passive exposure

Christina Tsitsimpikou et al. Biomed Rep. 2016 Dec.

Abstract

Stanozolol is a synthetic heterocyclic steroid with anabolic and androgenic properties, which has been abused by several high-profile professional athletes. Stanozolol is also used in veterinary medicine to increase appetite, cause weight gain and treat certain types of anemia. The detection of stanozolol metabolites in human urine for doping control purposes depends on the analytical method applied. The most commonly applied methods in the World Anti-Doping Agency (WADA)-accredited doping control laboratories are gas chromatography/high-resolution mass spectrometry (GC/HRMS) or gas chromatography-mass spectrometry (GC/MS/MS). Recently, a new method has been published and validated that makes the detection of 3'-hydroxystanozolol glucuronide in urine possible in a concentration >50-fold less compared to the above-mentioned commonly used methods. It is common practice to administer breeding animals with steroid hormones in order to enhance their growth. Athletes who consume meat containing such hormone residues may be at risk of failing a sports drug test. A randomized study in the general population consuming meat should be conducted, monitoring the levels of 3'-OH-stanozolol glucoronide in human urine, in order to determine the threshold levels of passive exposure, if any, and therefore guarantee that any adverse analytical findings reported in the urine of athlete at a pg level correspond to stanozolol abuse for enhancing performance.

Keywords: 3′-OH-stanozolol glucuronide; animal breeding; doping; passive exposure; stanozolol.

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References

    1. Poelmans S, De Wasch K, De Brabander HF, van De Wiele M, Courtheyn D, van Ginkel LA, Sterk SS, Delahaut P, Dubois M, Schilt R, et al. Analytical possibilities for the detection of stanozolol and its metabolites. Anal Chim Acta. 2002;473:39–47. doi: 10.1016/S0003-2670(02)00672-4. - DOI
    1. Schänzer W, Opfermann G, Dönike M. Metabolism of stanozolol: Identification and synthesis of urinary metabolites. J Steroid Biochem. 1990;36:153–174. doi: 10.1016/0022-4731(90)90126-D. - DOI - PubMed
    1. Schänzer W, Delahaut P, Geyer H, Machnik M, Horning S. Long-term detection and identification of metandienone and stanozolol abuse in athletes by gas chromatography-high-resolution mass spectrometry. J Chromatogr B Biomed Appl. 1996;687:93–108. doi: 10.1016/S0378-4347(96)00187-9. - DOI - PubMed
    1. Schänzer W, Guddat S, Thomas A, Opfermann G, Geyer H, Thevis M. Expanding analytical possibilities concerning the detection of stanozolol misuse by means of high resolution/high accuracy mass spectrometric detection of stanozolol glucuronides in human sports drug testing. Drug Test Anal. 2013;5:810–818. doi: 10.1002/dta.1516. - DOI - PubMed
    1. De Brabander HF, De Wasch K, van Ginkel LA, Sterk SS, Blokland MH, Delahaut P, Taillieu X, Dubois M, Arts CJ, van Baak MJ, et al. Multi-laboratory study of the analysis and kinetics of stanozolol and its metabolites in treated calves. Analyst (Lond) 1998;123:2599–2604. doi: 10.1039/a805110k. - DOI - PubMed