Copper(II)-Controlled Molecular Glue for Mismatched DNA

Abstract

Isothermal hybridization of two DNA strands bearing three thymine-thymine (T:T) mismatches can be brought about in the presence of a stoichiometric amount of a bis-naphthalene macrocycle, 2,7-BisNP-NH. This process can be reverted by addition of a Cu^II salt due to formation of a dinuclear metal complex which does not bind to DNA. Subsequent sequestration of Cu^II releases the macrocycle and restores the hybridization state of DNA strands, thus allowing implementation of a fast fluorescent two-state DNA switch.

Keywords: DNA recognition; DNA switch; hybridization; macrocycles; metal complexes.