Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Mar;14(3):283-286.
doi: 10.1038/nmeth.4147. Epub 2017 Jan 23.

High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles

Michiel van de Waterbeemd  1   2 , Kyle L Fort  1   2 , Dmitriy Boll  3 , Maria Reinhardt-Szyba  3 , Andrew Routh  4   5 , Alexander Makarov  1   3 , Albert J R Heck  1   2
Affiliations

High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles

Michiel van de Waterbeemd et al. Nat Methods. 2017 Mar.

Abstract

Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8- to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.

PubMed Disclaimer

MeSH terms

Substances

LinkOut - more resources