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. 2016 Nov 1;110(11):670-672.
doi: 10.1093/trstmh/trw079.

Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures

Adul Dulsuk  1 , Suporn Paksanont  2 , Adisak Sangchankoom  3 , Peeraya Ekchariyawat  2 , Rungnapa Phunpang  1 , Yaowaruk Jutrakul  3 , Narisara Chantratita  1   2 , T Eoin West  2   4   5
Affiliations

Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures

Adul Dulsuk et al. Trans R Soc Trop Med Hyg. .

Abstract

Background: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis.

Methods: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016.

Results: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p<0.0001.

Conclusions: The IFA accurately expedites the diagnosis of melioidosis.

Keywords: Burkholderia pseudomallei; Bacteremia; Diagnosis; Immunofluorescent assay; Melioidosis; Thailand.

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