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. 2017 Jan 9;14(1):189-191.
doi: 10.1016/j.jor.2016.12.003. eCollection 2017 Mar.

Evaluation of rapid GeneXpert MTB/RIF method using DNA tissue specimens of vertebral bones in patients with suspected spondylitis TB

Muhammad Nasrum Massi  1 , Karya Triko Biatko  2 , Irda Handayani  3 , Muhammad Yogi Pratama  1 , Sari Septriani  4 , Gaby Maulida Nurdin  4 , Marina B Ali  4
Affiliations

Evaluation of rapid GeneXpert MTB/RIF method using DNA tissue specimens of vertebral bones in patients with suspected spondylitis TB

Muhammad Nasrum Massi et al. J Orthop. .

Abstract

Purpose: To detect Mycobacterium tuberculosis DNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects.

Methods and results: The rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%.

Conclusion: GeneXpert has a high sensitivity but low specificity value in this study.

Keywords: DNA M. tuberculosis; GeneXpert MTB/RIF; Specimen pus; Spondylitis TB.

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