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. 2016 Dec;54(4):450-454.
doi: 10.17113/ftb.54.04.16.4518.

Biological Treatment of Cyanide by Using Klebsiella pneumoniae Species

Nermin Hande Avcioglu  1 , Isil Seyis Bilkay  1
Affiliations

Biological Treatment of Cyanide by Using Klebsiella pneumoniae Species

Nermin Hande Avcioglu et al. Food Technol Biotechnol. 2016 Dec.

Abstract

In this study, optimization conditions for cyanide biodegradation by Klebsiella pneumoniae strain were determined to be 25 °C, pH=7 and 150 rpm at the concentration of 0.5 mM potassium cyanide in the medium. Additionally, it was found that K. pneumoniae strain is not only able to degrade potassium cyanide, but also to degrade potassium hexacyanoferrate(II) trihydrate and sodium ferrocyanide decahydrate with the efficiencies of 85 and 87.5%, respectively. Furthermore, this strain degraded potassium cyanide in the presence of different ions such as magnesium, nickel, cobalt, iron, chromium, arsenic and zinc, in variable concentrations (0.1, 0.25 and 0.5 mM) and as a result the amount of the bacteria in the biodegradation media decreased with the increase of ion concentration. Lastly, it was also observed that sterile crude extract of K. pneumoniae strain degraded potassium cyanide on the fifth day of incubation. Based on these results, it is concluded that both culture and sterile crude extract of K. pnemoniae will be used in cyanide removal from different wastes.

Keywords: Klebsiella pneumoniae; biodegradation; cyanide.

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Figures

Fig. 1
Fig. 1
Biodegradation of potassium cyanide by different K. pneumoniae strains (Kp1–Kp17)
Fig. 2
Fig. 2
Effect of incubation period on cyanide biodegradation by K. pneumoniae Kp2
Fig. 3
Fig. 3
Effect of initial cyanide concentration on cyanide biodegradation by K. pneumoniae Kp2
Fig. 4
Fig. 4
Effect of initial pH on cyanide biodegradation by K. pneumoniae Kp2
Fig. 5
Fig. 5
Effect of rotation speed on cyanide biodegradation by K. pneumoniae Kp2
Fig. 6
Fig. 6
Effect of temperature on cyanide biodegradation by K. pneumoniae Kp2
Fig. 7
Fig. 7
Effect of different ions on the growth (A600 nm) of K. pneumoniae Kp2 in cyanide biodegradation process
Fig. 8
Fig. 8
Effect of incubation period on cyanide biodegradation by different concentrations of sterile crude extract of K. pneumoniae Kp2
See this image and copyright information in PMC

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