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. 2016 Dec 9:16:13-24.
doi: 10.1016/j.nmni.2016.12.001. eCollection 2017 Mar.

Paenibacillus phocaensis sp. nov., isolated from the gut microbiota of a healthy infant

M Tidjani Alou  1 , J Rathored  1 , T T Nguyen  1 , C Andrieu  1 , C Couderc  1 , S Brah  2 , B A Diallo  3 , P-E Fournier  1 , D Raoult  4 , G Dubourg  1
Affiliations

Paenibacillus phocaensis sp. nov., isolated from the gut microbiota of a healthy infant

M Tidjani Alou et al. New Microbes New Infect. .

Expression of concern in

Abstract

Paenibacillus phocaensis sp. nov. strain mt24T (= CSUR P2238 = DSM 101777) is a Gram-negative, facultative anaerobic, spore-forming and motile bacilli. This strain was isolated from the stool sample of a healthy infant from Niger. Its genome was estimated to a size of 5 521 415 bp with a 53.54% GC content. It contains 4835 protein-coding genes and 89 RNAs, among which two were 16S rRNA genes. There were also 101 genes (2.09%) identified as ORFans.

Keywords: Culturomics; Paenibacillus phocaensis; genome; gut microbiota; taxonogenomics.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree highlighting position of Paenibacillus phocaensis strain mt24T (= CSUR P2238 = DSM 101777) relative to other close strains. Respective GenBank accession numbers for 16S rRNA genes are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences were obtained using maximum-likelihood method within MEGA6 software. Numbers at nodes are percentages of bootstrap values obtained by repeating analysis 1000 times to generate majority consensus tree. Clostridium felsineum strain NCIMB 10690 was used as outgroup. Scale bar represents 2% nucleotide sequence divergence.
Fig. 2
Fig. 2
Reference mass spectrum from Paenibacillus phocaensis strain mt24T (= CSUR P2238 = DSM 101777). Spectra from 12 individual colonies were compared and reference spectrum generated.
Fig. 3
Fig. 3
Reference mass spectrum from Paenibacillus phocaensis strain mt24T (= CSUR P2238 = DSM 101777). Spectra from 12 individual colonies were compared and reference spectrum generated.
Fig. 4
Fig. 4
Gram staining of Paenibacillus phocaensis strain mt24T (= CSUR P2238 = DSM 101777).
Fig. 5
Fig. 5
Transmission electron microscopy of Paenibacillus phocaensis strain mt24T (= CSUR P2238 = DSM 101777) using Tecnai G20 transmission electron microscope (FEI Company) at operating voltage of 60 kV. Scale bar = 500 nm.
Fig. 6
Fig. 6
Graphical circular map of chromosome. From outside to centre: genes on forward strain colored by COGs categories (only gene assigned to COGs), RNA genes (tRNAs green, rRNAs red), G+C content and G+C skew. COGs, Clusters of Orthologous Groups database.
Fig. 7
Fig. 7
Distribution of functional classes of predicted genes according to COGs of protein. COGs, Clusters of Orthologous Groups database.
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