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. 2016;6(3):247-254.
doi: 10.4314/ovj.v6i3.15. Epub 2016 Dec 19.

Proliferation, angiogenesis and differentiation related markers in compact and follicular-compact thyroid carcinomas in dogs

P Pessina  1 , V A Castillo  2 , D César  3 , I Sartore  1 , A Meikle  1
Affiliations

Proliferation, angiogenesis and differentiation related markers in compact and follicular-compact thyroid carcinomas in dogs

P Pessina et al. Open Vet J. 2016.

Abstract

Immunohistochemical markers (IGF-1, IGF-1R, VEGF, FGF-2, RARα and RXR) were evaluated in healthy canine thyroid glands (n=8) and in follicular-compact (n=8) and compact thyroid carcinomas (n=8). IGF-1, IGF-1R and VEGF expression was higher in fibroblasts and endothelial cells of compact carcinoma than in healthy glands (P < 0.05). Compared to follicular-compact carcinoma, compact carcinoma had higher IGF-1R expression in fibroblasts, and higher FGF-2 expression in endothelial cells (P < 0.05). RARα expression was higher in endothelial cells of compact carcinoma than in those of other groups (P < 0.05). The upregulation of these proliferation- and angiogenesis-related factors in endothelial cells and/or fibroblasts and not in follicular cells of compact carcinoma compared to healthy glands supports the relevance of stromal cells in cancer progression.

Keywords: Canine; Histology; Immunohistochemistry; Thyroid carcinoma.

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Figures

Fig. 1
Fig. 1
Immunoreactivity of insulin-like growth factor-1 (IGF-1, A) and insulin-like growth factor-1 receptor (IGF-1R, B) in thyroid compact carcinoma. Cytoplasmic labeling in follicular cells is shown. The asterisk (*) shows expression in healthy thyroid tissue. All images were captured at a magnification of 400×.
Fig. 2
Fig. 2
Vascular endothelial growth factor (VEGF, A) and fibroblast growth factor-2 (FGF-2, B) cytoplasmic labeling in thyroid compact carcinoma. The asterisk (*) shows expression in healthy thyroid tissues. All images were captured at a magnification of 400×.
Fig. 3
Fig. 3
Cytoplasmic immunolabeling of retinoic acid receptor alpha (RARα, A) and retinoid X receptor (RXR, B) in thyroid compact carcinoma. All images were captured at a magnification of 400×.
Fig. 4
Fig. 4
Immunolabeling intensity of insulin-like growth factor-1 (IGF-1, A) and insulin-like growth factor-1 receptor (IGF-1R, B). Data are expressed as the least square mean ± pooled S.E.M. Different letters within the same cell type indicate differences, P < 0.05.
Fig. 5
Fig. 5
Immunolabeling intensity of vascular endothelial growth factor (VEGF, A) and fibroblast growth factor-2 (FGF-2, B). Data are expressed as the least square mean ± pooled S.E.M. Different letters within the same cell type indicate differences, P < 0.05.
Fig. 6
Fig. 6
Immunolabeling intensity of retinoic acid receptor alpha (RARα, A) and retinoid X receptor (RXR, B). Data are expressed as the least square mean ± pooled S.E.M. Different letters within the same cell type indicate differences, P < 0.05.
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References

    1. Barber LG. Thyroid tumors in dogs and cats. Vet. Clin. North Am. Small Anim. Pract. 2007;37(4):755–773. - PubMed
    1. Bissell MJ, Radisky D. Putting tumors in context. Nat. Rev. Cancer. 2001;1(1):46–54. - PMC - PubMed
    1. Boelaert K, McCabe CJ, Tannahill LA, Gittoes NJ, Holder RL, Watkinson JC, Bradwell AR, Sheppard MC, Franklyn JA. Pituitary tumor transforming gene and fibroblast growth factor-2 expression: potential prognostic indicators in differentiated thyroid cancer. J. Clin. Endocrinol. Metab. 2003;88(5):2341–2347. - PubMed
    1. Boos A, Meyer W, Schwarz R, Grunert E. Immunohistochemical assessment of oestrogen receptor and progesterone receptor distribution in biopsy samples of the bovine endometrium collected throughout the oestrous cycle. Anim. Reprod. Sci. 1996;44:11–21.
    1. Bouziges F, Simo P, Simon-Assmann P, Haffen K, Kedinger M. Altered deposition of basement membrane molecule in co-culture of colonic cancer cells and fibroblast. Int. J. Cancer. 1991;48(1):101–108. - PubMed

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