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Review
. 2017:1527:1-26.
doi: 10.1007/978-1-4939-6625-7_1.

Large-Scale Transcriptome Analysis

David Weaver  1   2 , Kathirvel Gopalakrishnan  2   3 , Bina Joe  4   5   6
Affiliations
Review

Large-Scale Transcriptome Analysis

David Weaver et al. Methods Mol Biol. 2017.

Abstract

Genomic variants identified to be linked with complex traits such as blood pressure are fewer in coding sequences compared to noncoding sequences. This being the case, there is an increasing need to query the expression of genes at a genome scale to then correlate the cause of alteration in expression to the function of variants regardless of where they are located. To do so, quering transcriptomes using microarray technology serves as a good experimental tool. This Chapter presents the basic methods needed to conduct a microarray experiment and points to resources avaiable online to complete the analysis and generate data regarding the transcriptomic status of a tissue sample relevant to hypertension.

Keywords: Chip; Microarray; array; blood pressure; lncRNA; mRNA.

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