Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2017 Jan 20;7(1):7.
doi: 10.3390/diagnostics7010007.

Salivary Diagnostics-Point-of-Care diagnostics of MMP-8 in dentistry and medicine

Nilminie Rathnayake  1 , Dirk-Rolf Gieselmann  2 , Anna Maria Heikkinen  3 , Taina Tervahartiala  4 , Timo Sorsa  5   6
Affiliations
Review

Salivary Diagnostics-Point-of-Care diagnostics of MMP-8 in dentistry and medicine

Nilminie Rathnayake et al. Diagnostics (Basel). .

Abstract

Human saliva is an easily accessible biological fluid and contains a variety of disease-related biomarkers, which makes it a potential diagnostic medium. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires the development of non-invasive screening and diagnostic technologies, and is among the main goals of oral fluid researchers. The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth-rinse, gingival crevicular fluid (GCF) and peri-implantitis sulcular fluid (PISF)) is demanding. Several factors influence their expression and release; these factors include the intracellular location, the molecular size and the flow characteristics of the biological fluid. The type of saliva/oral fluid utilized for the diagnostics affects the analysis. High sensitivity together with sophisticated methods and techniques are essential to get a useful outcome. We describe here a recently developed mouth-rinse that is practical, convenient and inexpensive, as well as PISF chair-side/point of care (PoC) lateral-flow active matrix metalloproteinase (aMMP-8) immunoassays to detect, predict and monitor the course and treatment of periodontitis and peri-implantitis.

Keywords: diagnostic test; peri-implantitis; periodontitis; point-of-care; systemic diseases.

PubMed Disclaimer

Conflict of interest statement

Timo Sorsa is inventor of US-Patents 5652227, 5866432 and 6143476.

Figures

Figure 1
Figure 1
Components, properties and secretion of human whole saliva.
Figure 2
Figure 2
Salivary test kit used to detect the presence of aMMP-8 in periodontitis patients.
Figure 3
Figure 3
Two lines (Control line; C-line and Test line; T-line) due to elevated saliva aMMP-8 indicate elevated risk for periodontitis.
Figure 4
Figure 4
ImplantSafe rapid test kit to detect peri-implantitis.
Figure 5
Figure 5
(a) Demonstrate the clinical and radiographic features of peri-implantitis and use of ImplantSafe; (b) Two clear lines indicate elevated risk for peri-implantitis. A positive test result: both control (C) line and test (T) line are visible, reveals elevated collagenolytic activity.
See this image and copyright information in PMC

References

    1. Veerman E.C., van den Keybus P.A., Vissink A., Nieuw Amerongen A.V. Human glandular salivas: Their separate collection and analysis. Eur. J. Oral Sci. 1996;104:346–352. doi: 10.1111/j.1600-0722.1996.tb00090.x. - DOI - PubMed
    1. Lee Y.H., Wong D.T. Saliva: An emerging biofluid for early detection of diseases. Am. J. Dent. 2009;22:241–248. - PMC - PubMed
    1. Rathnayake N., Åkerman S., Klinge B., Lundegren N., Jansson H., Tryselius Y., Sorsa T., Gustafsson A. Salivary Biomarkers for Detection of Systemic Diseases. PLoS ONE. 2013;8:e61356. doi: 10.1371/journal.pone.0061356. - DOI - PMC - PubMed
    1. Seymour G.J., Gemmell E. Cytokines in periodontal disease: Where to from here? Acta Odontol. Scand. 2001;59:167–173. doi: 10.1080/000163501750266765. - DOI - PubMed
    1. Kaufman E., Lamster I.B. The diagnostic applications of saliva a review. Crit. Rev. Oral. Biol. Med. 2002;13:197–212. doi: 10.1177/154411130201300209. - DOI - PubMed