doi: 10.1007/BF00484088.
Rudimentary locus of Drosophila melanogaster: partial purification of a carbamylphosphate synthase--aspartate transcarbamylase--dihydroorotase complex
- PMID: 28118
- DOI: 10.1007/BF00484088
Item in Clipboard
Rudimentary locus of Drosophila melanogaster: partial purification of a carbamylphosphate synthase--aspartate transcarbamylase--dihydroorotase complex
Biochem Genet.
1978 Apr.
Abstract
Glutamine-dependent CPSase, ATCase, and DHOase from Drosophila, the first three enzymes in pyrimidine biosynthesis, show coordinate variation in activity throughout development. The three activities were highest in first instar larvae and decreased as development proceeded. The three activities cosediment in sucrose gradients as a single peak with a relative sedimentation coefficient of approximately 30S. CPSase, ATCase, and DHOase copurify during (NH4)2SO4 fractionation and during DEAE-cellulose and hydroxylapatite chromatography.
Similar articles
-
Genetic and biochemical properties of revertants at the rudimentary locus in Drosophila melanogaster.Mol Gen Genet. 1981;183(2):270-6. doi: 10.1007/BF00270628. Mol Gen Genet. 1981. PMID: 6120439
-
Modification of the aggregation state of the multifunctional enzyme complex catalyzing the first steps in pyrimidine biosynthesis in the course of development of Drosophila melanogaster.Dev Biol. 1981 May;84(1):157-63. doi: 10.1016/0012-1606(81)90379-1. Dev Biol. 1981. PMID: 6114002 No abstract available.
-
Purification of a multifunctional protein bearing carbamyl-phosphate synthase, aspartate transcarbamylase, and dihydroorotase enzyme activities from mutant hamster cells.Methods Enzymol. 1978;51:121-34. doi: 10.1016/s0076-6879(78)51020-3. Methods Enzymol. 1978. PMID: 29210 No abstract available.
-
CAD gene sequence and the domain structure of the mammalian multifunctional protein CAD.Biochem Soc Trans. 1993 Feb;21(1):186-91. doi: 10.1042/bst0210186. Biochem Soc Trans. 1993. PMID: 8095469 Review. No abstract available.
-
[Gene amplification].Tanpakushitsu Kakusan Koso. 1984 Mar;29(3):152-63. Tanpakushitsu Kakusan Koso. 1984. PMID: 6425988 Review. Japanese. No abstract available.
Cited by
-
Genetic and biochemical properties of revertants at the rudimentary locus in Drosophila melanogaster.Mol Gen Genet. 1981;183(2):270-6. doi: 10.1007/BF00270628. Mol Gen Genet. 1981. PMID: 6120439
-
The action of the notchlocus in Drosophila melanogaster. II. Biochemical effects of recessive lethals on mitochondrial enzymes.Genetics. 1981 Sep;99(1):65-74. doi: 10.1093/genetics/99.1.65. Genetics. 1981. PMID: 6804303 Free PMC article.
-
Two non-gypsy rudimentary mutations and their suppression by mutations of suppressor of Hairy-wing in Drosophila.Mol Gen Genet. 1992 Nov;235(2-3):441-9. doi: 10.1007/BF00279391. Mol Gen Genet. 1992. PMID: 1281517
-
Analysis of the phenotypes exhibited by rudimentary-like mutants of Drosophila melanogaster.Biochem Genet. 1982 Aug;20(7-8):607-19. doi: 10.1007/BF00483960. Biochem Genet. 1982. PMID: 6814416
-
Construction of a cDNA to the hamster CAD gene and its application toward defining the domain for aspartate transcarbamylase.Mol Cell Biol. 1985 Jul;5(7):1735-42. doi: 10.1128/mcb.5.7.1735-1742.1985. Mol Cell Biol. 1985. PMID: 2862577 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Molecular Biology Databases