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. 2017 Feb;18(2):187-193.
doi: 10.15252/embr.201643717. Epub 2017 Jan 24.

Of mice and CRISPR: The post-CRISPR future of the mouse as a model system for the human condition

Edison T Liu  1 , Ewelina Bolcun-Filas  1 , David S Grass  1 , Cathleen Lutz  1 , Steve Murray  1 , Lenny Shultz  1 , Nadia Rosenthal  1
Affiliations

Of mice and CRISPR: The post-CRISPR future of the mouse as a model system for the human condition

Edison T Liu et al. EMBO Rep. 2017 Feb.

Abstract

The mouse has been a major model system for biomedical research. Gene editing technologies will further increase its importance for studying the human condition and human diseases.

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Figures

Figure 1
Figure 1. Conventional gene targeting in ES cells versus CRISPR/Cas9‐mediated gene editing in zygotes
Generation of genetically engineered mouse models via ES cell targeting can take an average of 1 year and involves many intermediate steps. In contrast, CRISPR/Cas9 simplifies and accelerates the production of mice carrying the desired mutation (N1). This is achieved by eliminating the need to generate targeted ES cell line. In some cases, CRISPR/Cas9‐mediated embryo editing also produces homozygous mutants, which can add the additional benefit of preliminary phenotype assessment.
See this image and copyright information in PMC

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