Differential effect of ethanol on muscarinic cholinergic binding to rat and locust neural membranes

Abstract

We have compared the effect of ethanol, a membrane perturbant, on the muscarinic binding sites in neural membranes from a vertebrate (rat) and an insect (locust). The binding of the muscarinic antagonist [3H]quinuclidinyl benzilate ([3H]QNB) to both rat and locust neural membranes was inhibited by ethanol at 10-500 mM concentrations; but this inhibition was greater in the locust. Ethanol (500 mM) increased the apparent dissociation constant (KD') of [3H]QNB binding to rat membranes from 0.13 +/- 0.01 nM in control to 0.20 +/- 0.02 nM; there was also an small but significant reduction in the number of binding sites Bmax. In locust, 500 mM ethanol reduced the Bmax of [3H]QNB binding from 590 +/- 30 in control to 320 +/- 40 pmol/g protein; no significant alteration in the KD was detected. The dissociation rate constant (koff) of [3H]QNB increased from 0.020 +/- 0.003 in controls to 0.031 +/- 0.004 (min-1) in the presence of 500 mM ethanol, the association rate constant (Kon) did not change significantly. In locust, 500 mM ethanol did not affect either Kon or Koff. Competition experiments revealed that the binding affinities of both the agonist carbamylcholine and the antagonist atropine to the rat membranes were reduced in the presence of ethanol. In contrast, ethanol caused no alteration in the binding affinities of these ligands to the locust membranes. This differential effect of ethanol on rat and locust muscarinic binding suggests a difference in the hydrophobic domains and/or the membrane interactions of the muscarinic receptors in the two species.