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. 2017 Jan;13(1):127-130.
doi: 10.3892/etm.2016.3959. Epub 2016 Dec 6.

Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia

Junguo Li  1 , Li Zhang  2 , Bing Li  3
Affiliations

Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia

Junguo Li et al. Exp Ther Med. 2017 Jan.

Retraction in

Abstract

The aim of the present study was to investigate the possible mechanism of Janus kinase (JAK)-signal transduction and activator of transcription (STAT)/PSMβ3 signaling in the occurrence of asthenozoospermia. We examined seminal fluid samples from 30 cases of asthenozoospermia and 30 healthy controls. Sperm was collected using the Percoll density gradient centrifugation method. The expression of JAK, STAT and PSMβ3 mRNA was assessed by reverse-transcription quantitative PCR and the protein levels of p-JAK, p-STAT and PSMβ3 were measured by western blot analysis. The PSMβ3 mRNA and protein expression levels were also measured after application of a JAK inhibitor, AG-490, to the control group, with a FITC-labeled monoclonal rabbit anti-human PSMβ3 primary antibody. The cells were observed under a laser confocal microscope. The mRNA levels of JAK, STAT and PSMβ3 in asthenozoospermia were decreased significantly (P<0.05). The protein levels of p-JAK, p-STAT and PSMβ3 in asthenozoospermia were also reduced and the differences were statistically significant (P<0.05). The PSMβ3 mRNA and protein expression levels were decreased in the control group after treatment with the JAK inhibitor, and levels were approximately equal to those of the asthenozoospermia group. PSMβ3 was mainly expressed in round-headed sperm, and less in asthenozoospermia. In conclusion, the JAK-STAT/PSMβ3 signaling transduction pathway may be involved in the pathogenic mechanism of asthenozoospermia.

Keywords: JAK-STAT/PSMβ3; asthenozoospermia; real-time quantitative polymerase chain reaction; western blot analysis.

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Figures

Figure 1.
Figure 1.
Expression of JAK, STAT, and PSMβ3 mRNA in the different groups. (A) PCR was performed to detect JAK, STAT and PSMβ3 mRNA. (B) Quantification of PCR data in A. JAK, Janus kinase; STAT, signal transduction and activator of transcription; PCR, polymerase chain reaction.
Figure 2.
Figure 2.
Expression of JAK, STAT and PSMβ3 protein in the different groups. (A) Western blot analysis was performed to detect the protein levels of JAK, STAT and PSMβ3. (B) Quantification of western blot analysis data in A. JAK, Janus kinase; STAT, signal transduction and activator of transcription.
Figure 3.
Figure 3.
Immunofluorescent staining of PSMβ3. (A) Asthenozoospermia group (PSMβ3 is shown in red). (B) Control group.
See this image and copyright information in PMC

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