High Expression of LINC01420 indicates an unfavorable prognosis and modulates cell migration and invasion in nasopharyngeal carcinoma

doi:10.7150/jca.16819

. 2017 Jan 1;8(1):97-103.

doi: 10.7150/jca.16819. eCollection 2017.

High Expression of LINC01420 indicates an unfavorable prognosis and modulates cell migration and invasion in nasopharyngeal carcinoma

Liting Yang¹, Yanyan Tang², Yi He³, Yumin Wang², Yu Lian⁴, Fang Xiong⁵, Lei Shi⁶, Shanshan Zhang⁵, Zhaojian Gong⁶, Yujuan Zhou⁷, Qianjin Liao³, Ming Zhou¹, Xiaoling Li², Wei Xiong¹, Yong Li⁸, Guiyuan Li¹, Zhaoyang Zeng¹, Can Guo¹

Affiliations

¹ The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
² The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.
³ The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
⁴ The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.
⁵ The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁶ The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁷ Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
⁸ The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.

PMID: 28123602
PMCID: PMC5264044
DOI: 10.7150/jca.16819

High Expression of LINC01420 indicates an unfavorable prognosis and modulates cell migration and invasion in nasopharyngeal carcinoma

Liting Yang et al. J Cancer. 2017.

. 2017 Jan 1;8(1):97-103.

doi: 10.7150/jca.16819. eCollection 2017.

Authors

Affiliations

¹ The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
² The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.
³ The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
⁴ The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.
⁵ The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁶ The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
⁷ Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
⁸ The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, China;; The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China;; Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.

PMID: 28123602
PMCID: PMC5264044
DOI: 10.7150/jca.16819

Abstract

Recent studies demonstrated that long non-coding RNAs (lncRNAs) deregulated in many cancer tissues including nasopharyngeal carcinoma (NPC) and had critical roles in cancer progression and metastasis. In this study, we aimed to assess a lncRNA LINC01420 expression in NPC and explore its role in NPC pathogenesis. Our research revealed that the expression level of LINC01420 in NPC tissues were higher than nasopharyngeal epithelial (NPE) tissues. Moreover, NPC patients with high LINC01420 expression level showed poor overall survival. Knockdown LINC01420 inhibited NPC cell migration and invasion in vitro. In summary, LINC01420 may play a critical role in NPC progression and may serve as a potential prognostic biomarker in NPC patients.

Keywords: lncRNAs; nasopharyngeal carcinoma.

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Conflict of interest statement

The authors declare that there are no conflicts of interest in this work.

Figures

**Figure 1**
*LINC01420* is highly expressed in head and neck cancer. *LINC01420* significantly highly expressed in Gene Expression Profiling (GEP) datasets GSE6791 (Normal, n = 14; Tumor, n = 42, p = 0.041) and GSE30784 (Normal, n = 62; Tumor, n= 167, p = 0.001).

**Figure 2**
*LINC01420* is highly expressed in NPC tissues and cell lines. (A) *LINC01420* expression was higher in NPC samples (Tumor, n = 26) than that in non-cancerous tissues (Normal, n = 10). (B) *LINC01420* expression was significantly increased in NPC cell lines (HNE1, HK1, HNE2, and 5-8F) compared with NP69, a normal human nasopharyngeal epithelium cell line.

**Figure 3**
The relationship between *LINC01420* expression and clinicopathological features in NPC patients. (A) *LINC01420* expression measured by in *situ* hybridization in paraffin embedded NPC biopsies. (B) *LINC01420* expression was highly expressed in NPC cells (Tumor) compared to surrounded non-cancer NPE cells (Normal, p = 0.02). (C) High *LINC01420* expression was associated with distant metastasis (p = 0.026). (D) More male NPC patients have high *LINC01420* expression than female patients (p = 0.029).

**Figure 4**
Kaplan-Meier survival curves of patients with NPC based on *LINC01420* expression. Patients with high *LINC001420* expression had a significantly unfavorable prognosis than those in low expression group (p = 0.015).

**Figure 5**
*LINC01420* Knockdown suppressed tumor cell migration and invasion *in vitro*. (A) siRNAs dramatically suppressed *LINC01420* expression in 5-8F cells (p < 0.001). (B and C) 5-8F cells were grown and transfected with *LINC01420* siRNAs, or scramble siRNA. Twenty-four hours after transfection, cells were subjected to a Transwell assay without or with Matrigel to measure migration or invasion capacity. *LINC01420* knockdown significantly inhibited 5-8F cell migration (p = 0.045) and invasion (p = 0.01).

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References

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[1] Kang H, Kiess A, Chung CH. Emerging biomarkers in head and neck cancer in the era of genomics. Nat Rev Clin Oncol. 2015;12:11–26. - PubMed

[2] Kang H, Kiess A, Chung CH. Emerging biomarkers in head and neck cancer in the era of genomics. Nat Rev Clin Oncol. 2015;12:11–26. - PubMed

[3] Zeng Z, Huang H, Zhang W. et al. Nasopharyngeal carcinoma: advances in genomics and molecular genetics. Sci China Life Sci. 2011;54:966–75. - PubMed

[4] Zeng Z, Huang H, Zhang W. et al. Nasopharyngeal carcinoma: advances in genomics and molecular genetics. Sci China Life Sci. 2011;54:966–75. - PubMed

[5] Xiong W, Zeng ZY, Xia JH. et al. A susceptibility locus at chromosome 3p21 linked to familial nasopharyngeal carcinoma. Cancer Res. 2004;64:1972–4. - PubMed

[6] Xiong W, Zeng ZY, Xia JH. et al. A susceptibility locus at chromosome 3p21 linked to familial nasopharyngeal carcinoma. Cancer Res. 2004;64:1972–4. - PubMed

[7] Zeng Z, Zhou Y, Zhang W. et al. Family-based association analysis validates chromosome 3p21 as a putative nasopharyngeal carcinoma susceptibility locus. Genet Med. 2006;8:156–60. - PubMed

[8] Zeng Z, Zhou Y, Zhang W. et al. Family-based association analysis validates chromosome 3p21 as a putative nasopharyngeal carcinoma susceptibility locus. Genet Med. 2006;8:156–60. - PubMed

[9] Zeng Z, Huang H, Huang L. et al. Regulation network and expression profiles of Epstein-Barr virus-encoded microRNAs and their potential target host genes in nasopharyngeal carcinomas. Sci China Life Sci. 2014;57:315–26. - PubMed

[10] Zeng Z, Huang H, Huang L. et al. Regulation network and expression profiles of Epstein-Barr virus-encoded microRNAs and their potential target host genes in nasopharyngeal carcinomas. Sci China Life Sci. 2014;57:315–26. - PubMed

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High Expression of LINC01420 indicates an unfavorable prognosis and modulates cell migration and invasion in nasopharyngeal carcinoma

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High Expression of LINC01420 indicates an unfavorable prognosis and modulates cell migration and invasion in nasopharyngeal carcinoma

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