Effect of cytosine arabinoside on rat cerebral explants: fibroblasts and reactive microglial cells as the primary cellular barrier to neurite growth

Abstract

Rat cerebral explants were cultured in the presence or absence of the mitotic inhibitor cytosine arabinoside (AraC) to investigate whether reports of increased neurite outgrowth may be primarily related to a decreased incidence of fibroblastic-reactive microglial cells. Treated explants were exposed to AraC from 7 to 10 days in vitro (DIV) and processed for transmission electron microscopy at 18 DIV. Morphometric analysis of the outgrowth zone revealed a statistically significant decrease in the incidence of fibroblastic-reactive microglial cells (from 12 to 0%), and a significant increase in the incidence of protoplasmic astrocytic-epithelial cells (from 82 to 96%), for AraC-treated explants compared to controls. In contrast, the incidence of fibrous astrocytes was similar to that of control explants (4 and 6%, respectively). Thus, it appears that AraC may primarily enhance neurite growth by curtailing the proliferation of fibroblastic-reactive microglial cells. These results suggest that fibroblastic-reactive microglial cells, rather than fibrous astrocytes, may constitute the primary cellular barrier to neurite growth.