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. 2017 Mar 4;12(3):e1284726.
doi: 10.1080/15592324.2017.1284726.

Protein import-independent functions of Tic56, a component of the 1-MDa translocase at the inner chloroplast envelope membrane

Birgit Agne  1 , Daniel Köhler  1 , Sacha Baginsky  1
Affiliations

Protein import-independent functions of Tic56, a component of the 1-MDa translocase at the inner chloroplast envelope membrane

Birgit Agne et al. Plant Signal Behav. .

Abstract

Tic56 is an essential subunit of a 1-MDa protein complex at the inner chloroplast envelope membrane that comprises Tic100, Tic214 and the protein conducting channel protein Tic20-I. The complex was characterized as the "general protein import translocase" because mutants in either of its subunits have a severe growth phenotype and fail to assemble a photosynthetic machinery. In a recent publication we show that the albino phenotype of tic56-1 mutants results at least in part from a defect in ribosome assembly and a deficiency in plastid translation. We furthermore could not detect any impairment of protein import activity with plastids from tic56-3 mutants, despite a lack of full-length Tic56 and a decreased abundance of other 1-MDa complex subunits. These findings suggest that the 1-MDa complex consists of subunits that have functions other than protein import.

Keywords: Chloroplast; protein import; quantitative proteomics; translocon at the inner chloroplast envelope membrane.

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Erratum for

  • Addendum to: D Köhler, S Helm, B Agne, S Baginsky.. Importance of translocon subunit Tic56 for rRNA processing and chloroplast ribosome assembly. Plant Physiol. 2016; 172: 2429-2444, PMID:27733515, http://dx.doi.org/10.1104/pp.16.01393. doi: 10.1104/pp.16.01393

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