Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Nov;86(21):8472-6.
doi: 10.1073/pnas.86.21.8472.

A nuclear protein associated with human cancer cells binds preferentially to a human repetitive DNA sequence

M L Law  1 , J Z Gao, T T Puck
Affiliations

A nuclear protein associated with human cancer cells binds preferentially to a human repetitive DNA sequence

M L Law et al. Proc Natl Acad Sci U S A. 1989 Nov.

Abstract

A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequence; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster cell line CHO-K1. Gel retardation assay confirmed the specificity of the protein-DNA binding between Rp66 and LRS. In a histiocytic lymphoma human cell line, U937, that can be induced to differentiate in the presence of phorbol ester, this binding disappeared after cell differentiation. These together with other results cited suggest a regulatory role for these repetitive sequences in the human genome, with particular application to cancer.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Annu Rev Biochem. 1986;55:631-61 - PubMed
    1. Biochem J. 1986 Feb 15;234(1):1-11 - PubMed
    1. Mol Cell Biol. 1987 Jun;7(6):2148-54 - PubMed
    1. J Cell Biol. 1987 Jul;105(1):117-25 - PubMed
    1. Nature. 1987 Jul 30-Aug 5;328(6129):445-9 - PubMed

Publication types

MeSH terms