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. 2017 Jan 30:7:41419.
doi: 10.1038/srep41419.

Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol

Jingyun Wang  1   2 , Lei Zhang  2 , Youju Huang  2 , Anirban Dandapat  3 , Liwei Dai  2 , Ganggang Zhang  1   2 , Xuefei Lu  2 , Jiawei Zhang  2 , Weihua Lai  1 , Tao Chen  2
Affiliations

Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol

Jingyun Wang et al. Sci Rep. .

Abstract

The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip (ICTS). Unlike conventional ICTS assay usually uses single-component, solid gold nanoparticles as labeled probes, in our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS was successfully developed for the detection of clenbuterol (CLE). The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were synthesized by varying the volume ratio of [HAuCl4]:[Ag NPs] via the galvanic replacement reaction. The surface of hollow Ag-Au NPs was functionalized with 11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE monoclonal antibody. Overall size of the Au-Ag NPs, size of the holes within individual NPs and also Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection signals and the quantitative data. The sensitivity of optimized hollow Au-Ag NPs probes has been achieved even as low as 2 ppb in a short time (within 15 min), which is superior over the detection performance of conventional test strip using Au NPs. The optimized hollow Au-Ag NPs labeled test strip can be used as an ideal candidate for the rapid screening of CLE in food samples.

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Conflict of interest statement

The authors declare no competing financial interests.

Figures

Figure 1
Figure 1
(a) The synthesis and surface modification of hollow Au-Ag NPs; (b) Schematic illustration of the detection of CLE using hollow Au-Ag NPs-labeled ICTS.
Figure 2
Figure 2. TEM images of Ag NPs (a) and hollow Ag-Au NPs in different growth stages (b–f), the Ag/Au ratios in images (b–f) are about 12.69:1, 4.78:1, 1.77:1, 0.98:1, and 0.46:1, respectively.
Figure 3
Figure 3. The color changes of Ag-Au porous NPs in the galvanic replacement reaction (a–f) after the addition of different volumes of 0.01 M HAuCl4 from 0, 12, 24, 36, 48 to 50 μL.
Figure 4
Figure 4. UV/vis absorption spectra of Ag NPs (a) and hollow Ag-Au NPs with different Ag/Au/ratio (b–f): 12.69:1, 4.78:1, 1.77:1, 0.98:1, and 0.46:1.
Figure 5
Figure 5. Detection of CLE samples (from 0 to 20 ppb) using Ag NPs (a), Ag-Au porous NPs (b), and Au NPs (c) labeled ICTS.
Figure 6
Figure 6. The optical density on the T line in the detection of different concentrations of CLE using Ag NPs and hollow Ag-Au NPs (a–f), and Au NPs (g).
Figure 7
Figure 7. The relationship between the detection limit of CLE and Ag-Au porous NPs with different Ag/Au ratio.
See this image and copyright information in PMC

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