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. 2017 Jan;108(1):81-83.
doi: 10.5935/abc.20160200.

The CRISPR/Cas9 System and the Possibility of Genomic Edition for Cardiology

[Article in Portuguese, English]
Marcela Corso Arend  1 , Jessica Olivaes Pereira  1 , Melissa Medeiros Markoski  1
Affiliations

The CRISPR/Cas9 System and the Possibility of Genomic Edition for Cardiology

[Article in Portuguese, English]
Marcela Corso Arend et al. Arq Bras Cardiol. 2017 Jan.
No abstract available

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Conflict of interest statement

Potential Conflict of Interest No potential conflict of interest relevant to this article was reported.

Figures

Figure 1
Figure 1
CRISPR/Cas9 system - target recognition mechanism. The guide RNA is designed to recognize the target sequence to be modified in the RNA and introduce modifications. When the pairing of nitrogenized bases occurs (due to the annealing of the target sequence with the region of the guide RNA protospacer), some modifications are added (represented hereby the circle) and the Cas9 enzyme is activated, causing breaks to the DNA double strand (where there are pairing flaws due to the mutations introduced). The breaks activate the intracellular repair systems that remake the double strand, accepting the modifications from the guide RNA. The new mutations generally cause flaws in the sequence and generate non-functional proteins. But the mechanism can also be used to correct mutations originally present in the DNA and generate functional proteins.
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