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. 2017 Mar;32(1):103-105.
doi: 10.1007/s12291-016-0577-6. Epub 2016 May 18.

Prevalence of Factor V Leiden-G1691A and MTHFR-C677T Thrombosis Gene Modifier in Iron Deficiency Anemia: A Pathophysiological Effect in Indian Isolates

S K Pandey  1 , S Pandey  1 , R M Mishra  2 , M Indurkar  3
Affiliations

Prevalence of Factor V Leiden-G1691A and MTHFR-C677T Thrombosis Gene Modifier in Iron Deficiency Anemia: A Pathophysiological Effect in Indian Isolates

S K Pandey et al. Indian J Clin Biochem. 2017 Mar.

Abstract

Normal iron levels are required to prevent thrombocytosis by inhibiting thrombopoiesis. Thrombocytosis is usually associated with a mild iron deficiency and is the result of a lack of inhibition of thrombopoiesis. Study participants were 430 iron deficiency anemia (IDA) patients. Ten (10) mL of venous blood were collected for the subjects. Ferritin analysis was done by ELISA method while Hemogram analysis was done by auto-analyzer. Factor V Leiden, PRTG20210A, and MTHFR C677T genotype analysis was performed by PCR-RFLP method. Among the patients, 9 were heterozygous (G>A) and 2 were homozygous (A>A) carrier of FV Leiden; while 20 were heterozygous (C>T) and 3 were homozygous (T>T) for MTHFR polymorphism. None of the patient was identified with PT mutation. Patients with thrombosis gene marker had lower hemoglobin, mean corpuscular volume, mean corpuscular haemoglobin levels, and mean corpuscular hemoglobin concentration than patients without thrombosis gene marker. Serum ferritin was elevated in subject with the absence of thrombosis gene markers. Our data suggest a high impact of inherited hypercoagulability risk factors in the pathogenesis of IDA and its complications.

Keywords: Factor V Leiden; IDA; MTHFR; Phenotype.

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Conflict of interest statement

None.

Figures

Fig. 1
Fig. 1
a Agarose gel picture of FV Leiden gene amplification. b Factor five Leiden mutations (lane 3, 6 heterozygous)
Fig. 2
Fig. 2
a Agarose gel picture of MTHFR gene amplification. b Restriction digestion for MTHFR (lane 2, 5, 8 are heterozygous and lane 7 is homozygous)
See this image and copyright information in PMC

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