Modulation of sensitivity to natural killer cell lysis after in vitro explantation of a mouse lymphoma

Abstract

On the basis of studies indicating that natural killer (NK) cells of the mouse can selectively kill certain syngenetic, allogeneic, and xenogeneic tumor cells in short-term Cr release assays and that cell lines established in vitro are more sensitive than the corresponding ascites tumor cells passaged in vivo, the kinetics of the modulation to increased sensitivity was studied after in vitro explanation of the A/Sn mouse-derived YAC ascites lymphoma. Sensitivity to NK lysis appeared after 3 weeks of culturing and reached the level of the continuously cultured line after 2 months. With the more sensitive competition assay, a change could be demonstrated as early as 2--24 hours of culture. The expression of the Moloney murine leukemia virus-determined, cell-surface antigen, measured by quantitative absorption with intact cells, increased in parallel with the NK sensitivity. In contrast, the H-2 alloantigen concentration decreased during in vitro culture.