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. 2013 Mar 26;8(1):20596.
doi: 10.3402/ljm.v8i0.20596.

A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia

Rıdvan Cakmaz  1 , Oktay Büyükaşık, Nurettin Kahramansoy, Hayri Erkol, Cavit Cöl, Cetin Boran, Güler Buğdaycı
Affiliations

A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia

Rıdvan Cakmaz et al. Libyan J Med. .

Abstract

Introduction: There is no valid and reliable diagnostic test for early diagnosis of acute mesenteric ischemia (AMI). The aim of this study was to measure the plasma levels of diamine oxidase (DAO) and citrulline in AMI to gain insight into its early diagnosis.

Material and methods: A total of 21 Wistar albino rats were divided into three groups, that is, control group, short-term ischemia group, and prolonged ischemia group. The superior mesenteric artery was occluded for 15 min in the short-term ischemia group and for 12 h in the prolonged ischemia group. Twelve hours later, the experiment was terminated and plasma DAO and citrulline levels were measured. Intestinal tissue was evaluated for the histopathological changes.

Results: Compared to the control group, the short-term and prolonged ischemia groups showed significant increases in the plasma levels of DAO, whereas the plasma citrulline levels decreased significantly. Prolonged ischemia caused a larger increase in the plasma DAO levels and a larger decrease in the plasma citrulline levels compared to the short-term ischemia (p=0.011 and p=0.021, respectively). Intestinal damage was shown to develop more in the prolonged ischemia group (p=0.001).

Conclusion: In the early period of AMI, the plasma DAO levels increase while citrulline levels decrease, and the extent of these changes depends on the duration of ischemia.

Keywords: animal model; citrulline; diamine oxidase; experimental; intestines.

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Figures

Fig. 1
Fig. 1
Morphological changes of the intestine in the short-term ischemia group. Panel a demonstrates the score Grade 3 (*: minimal necrosis of the surface epithelium at the tip of the villus). Panel b shows the score Grade 4 with (#) necrosis at the tip of the villi (H-E, ×200).
Fig. 2
Fig. 2
Morphological changes of the intestine in the prolonged ischemia group. Panel a demonstrates the score Grade 5 with full thickness mucosal necrosis. Panel b shows the score Grade 6 with transmural necrosis (H-E, ×200).
See this image and copyright information in PMC

References

    1. Alhan E, Usta A, Cekiç A, Saglam K, TürkyIlmaz S, Cinel A. A study on 107 patients with acute mesenteric ischemia over 30 years. Int J Surg. 2012;10:510–3. - PubMed
    1. Gupta PK, Natarajan B, Gupta H, Fang X, Fitzgibbons RJ., Jr Morbidity and mortality after bowel resection for acute mesenteric ischemia. Surgery. 2011;150:779–87. - PubMed
    1. Acosta-Merida MA, Marchena-Gomez J, Hemmersbach-Miller M, Roque-Castellano C, Hernandez-Romero JM. Identification of risk factors for perioperative mortality in acute mesenteric ischemia. World J Surg. 2006;30:1579–85. - PubMed
    1. Acosta S. Epidemiology of mesenteric vascular disease: clinical implications. Semin Vasc Surg. 2010;23:4–8. - PubMed
    1. Newton WB, 3rd, Sagransky MJ, Andrews JS, Hansen KJ, Corriere MA, Goodney PP, et al. Outcomes of revascularized acute mesenteric ischemia in the American College of Surgeons National Surgical Quality Improvement Program database. Am Surg. 2011;77:832–8. - PMC - PubMed

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